In-Vitro and In-Silico Study of Anti-Cancer Properties of Methanolic Crude Extract from Cassava (Manihot esculenta Crantz) Leaves on Breast Cancer Cells (MCF-7)

Document Types

Paper Presentation

Research Theme (for Paper Presentation and Poster Presentation submissions only)

Food, Nutrition, and Health (FNH)

School Name

Quezon City Science High School

Track or Strand

Science, Technology, Engineering, and Mathematics (STEM)

Research Advisor (Last Name, First Name, Middle Initial)

Vasquez, Genevieve, C.

Start Date

23-6-2026 1:30 PM

End Date

23-6-2026 3:00 PM

Zoom Link/ Room Assignment

DLSU Manila Campus (In-person) - Brother Andrew Gonzalez Multipurpose Hall, 20th floor

Abstract/Executive Summary

Breast cancer is the most prominent type of cancer among women. Cassava (Manihot esculenta Crantz), a root vegetable native to tropical countries, contains phenolic acids that are said to exhibit anticancer activity. This study aims to evaluate the anticancer properties of cassava leaf extract on breast cancer and predict its putative compounds' binding affinity to target 5U2B, an estrogen receptor, via molecular docking. Liquid Chromatography Tandem Mass spectrometry (LCMS/MS) identified the compounds in each sequential fraction. MTT assays were performed on MCF-7 breast cancer cells and SD-RFP-MSC fibroblast cells to determine the cytotoxicity and therapeutic index of the extracts, and the most toxic fraction. Paired molecular docking predicted how well the identified compounds bind to 5U2B. The methanolic crude extract had the lowest absorption, comparable to positive controls, Vinblastine and 5-Fluorouracil. LCMS/MS showed that it contained 23 compounds with known anticancer effects and 32 with limited research against cancer. Ephedradine A and (25r)-Spirostan-3,12-dione had the most negative binding affinity score of -10.7 in Section E, Mode 0, indicating a strong exergonic reaction. The methanolic cassava extract has a significant effect against breast cancer cells and contains compounds with potential to be processed as anti-cancer agents.

Keywords

crude extract; cassava leaves; breast cancer; MTT assay; molecular docking

Statement of Originality

yes

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Jun 23rd, 1:30 PM Jun 23rd, 3:00 PM

In-Vitro and In-Silico Study of Anti-Cancer Properties of Methanolic Crude Extract from Cassava (Manihot esculenta Crantz) Leaves on Breast Cancer Cells (MCF-7)

Breast cancer is the most prominent type of cancer among women. Cassava (Manihot esculenta Crantz), a root vegetable native to tropical countries, contains phenolic acids that are said to exhibit anticancer activity. This study aims to evaluate the anticancer properties of cassava leaf extract on breast cancer and predict its putative compounds' binding affinity to target 5U2B, an estrogen receptor, via molecular docking. Liquid Chromatography Tandem Mass spectrometry (LCMS/MS) identified the compounds in each sequential fraction. MTT assays were performed on MCF-7 breast cancer cells and SD-RFP-MSC fibroblast cells to determine the cytotoxicity and therapeutic index of the extracts, and the most toxic fraction. Paired molecular docking predicted how well the identified compounds bind to 5U2B. The methanolic crude extract had the lowest absorption, comparable to positive controls, Vinblastine and 5-Fluorouracil. LCMS/MS showed that it contained 23 compounds with known anticancer effects and 32 with limited research against cancer. Ephedradine A and (25r)-Spirostan-3,12-dione had the most negative binding affinity score of -10.7 in Section E, Mode 0, indicating a strong exergonic reaction. The methanolic cassava extract has a significant effect against breast cancer cells and contains compounds with potential to be processed as anti-cancer agents.

https://animorepository.dlsu.edu.ph/conf_shsrescon/2026/BoA_FNH/13