Cytotoxic triterpenes and sterols from Pipturus arborescens (link) C.B. Rob.

Authors

Mariquit M. De Los Reyes, Salle University Science and Technology
Glen G. Oyong, De La Salle University-Manila
Virgilio D. Ebajo, De La Salle University-Manila
Vincent Antonio S. Ng, De La Salle University-Manila
Chien Chang Shen, National Research Institute of Chinese Medicine Taiwan
Consolacion Y. Ragasa, De La Salle University-Manila

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

Journal of Applied Pharmaceutical Science

Volume

5

Issue

11

First Page

23

Last Page

30

Publication Date

1-1-2015

Abstract

© 2015 Mariquit M. De Los Reyes et al. The triterpenes, squalene (1), friedelin (2) and a mixture of ursolic acid (3a) and oleanolic acid (3b) in a 2:3 ratio, and a mixture of ß-sitosterol (4a) and stigmasterol (4b) in a 2:1 ratio, obtained from the dichloromethane extract of Pipturus arborescens (Link) C.B. Rob., were evaluated for their anti-proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast- neonatal (HDFn) using the in vitro PrestoBlue® cell viability assay. The HCT-116 cell line was most susceptible to the compounds and mixtures tested. Triterpene 1 was most cytotoxic against HCT-116 and MCF-7 with IC50 values of 4.21 and 5.92 μg/mL, respectively. Triterpene 2 and the mixture of 3a and 3b were highly anti-proliferative against HCT-116 cells (IC50 of 1.22 and 1.66 μg/mL, respectively) and moderately inhibitory against MCF-7 cells (IC50 of 16.51 and 23.97 μg/mL, respectively). The mixture of 4a and 4b exhibited high cytotoxicity against HCT-116 cells (IC50 of 1.14 μg/mL). Compounds 1-4b showed the least activity against HT-29 cells (IC50 of 11.97 to 52.52 μg/mL). Cytotoxic effect was not observed against HDFn cells (>100 μg/mL). Comparing the effects of 1-4b on the two colon cancer cell lines, the IC50 values of 1-4b against HCT-116 were lower than those of HT-29.

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Digitial Object Identifier (DOI)

10.7324/JAPS.2015.501104

Recommended Citation

De Los Reyes, M. M., Oyong, G. G., Ebajo, V. D., Ng, V. S., Shen, C., & Ragasa, C. Y. (2015). Cytotoxic triterpenes and sterols from Pipturus arborescens (link) C.B. Rob.. Journal of Applied Pharmaceutical Science, 5 (11), 23-30. https://doi.org/10.7324/JAPS.2015.501104

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