Preliminary screening for microsatellite instability and loss of heterozygosity in the deleted in colorectal cancer (DCC) gene among Filipino patients with colorectal adenocarcinoma

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

Acta Medica Philippina

Volume

48

Issue

4

First Page

17

Last Page

22

Publication Date

2014

Publication Status

1

Abstract

Objective. This study aimed to detect the presence of microsatellite instability (MSI) and loss of heterozygosity (LOH) of the Deleted in Colorectal Cancer (DCO gene in normal and tumor tissues of Filipino colorectal cancer patients and examine its correlation with age, gender, tumor grade, tumor stage and site of lesion. Methods. Paired frozen normal and tumor tissues from thirty-nine (39) patients with colorectal adenocarcinoma were used in this study. Genomic DNA was extracted and amplified by polymerase chain reaction (PCR). Single strand conformation polymorphism polyacrylamide gel electrophoresis (SSCPPAGE) was used to determine MSI and restriction fragmentlength polymorphism (RFLP) was used to study LOH. Results. Based on our data, out of the 39 patients, 10 showed LOH of the DCC gene using the LOH markers VNTR, M2 and M3,while no MSI was detected in the samples using the MSI markersBAT25 and BAT26. Correlation with clinicopathological characteristics showed that there is significance for the site of lesion. The LOH has correlation with tumor samples from the colon but not with those from the rectum. in al al that Conclusion. Preliminary screening for MSI and LOH of the DCC gene shows that occurrence of colorectal cancer among Filipino patients can be correlated with LOH of the DCC gene but not with MSI. This may be the first study to examine the correlation or lack thereof of MSI and LOH of the DCC gene with colorectal cancer in a Filipino sample population.

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Disciplines

Cancer Biology | Cell and Developmental Biology | Life Sciences

Keywords

Colon (Anatomy)—Cancer; Cytology

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