Date of Publication
11-21-2025
Document Type
Master's Thesis
Degree Name
Master of Science in Biology
Subject Categories
Biology
College
College of Science
Department/Unit
Biology
Thesis Advisor
Mariquit M. De Los Reyes
Defense Panel Chair
John Martin S. Mondragon
Defense Panel Member
Gerard Anthony M. Espiritu
Charito T. Aranilla
Abstract (English)
End-stage kidney disease (ESKD) remains a major global health concern that only depends on two standard treatments, dialysis and kidney transplant. Extracellular matrix (ECM)-based hydrogels offer an innovative and versatile means of understanding cell behavior and disease progression, enabling the development of alternative treatment options for ESKD. This study assessed the physicochemical properties of hydrogels produced from porcine decellularized kidney (DK) ECM. Porcine kidneys were successfully decellularized using a novel protocol. There was an evident absence of native cells and a significant removal of DNA (~97.6%). There was also a significant elimination of ⍺-Gal (~95.8%), a major xenoantigen that can trigger an immune response. The resulting DKECM was used to fabricate hydrogels, which were subsequently crosslinked with different concentrations of glutaraldehyde (GA). Based on the results of physicochemical assessments, the 0.5 and 1.0% GA-crosslinked hydrogels have shown the desired hydrogel properties. They exhibited consistent stability, had a rough and porous architecture, and a wide range and high variability of pore sizes, suggesting their potential to simultaneously facilitate diffusion, cell migration, and vascular ingrowth. They also displayed higher swelling capacity, interconnected networks, and retained the essential ECM components, highlighting their potential as suitable biomaterials for tissue engineering applications, specifically as a 3D cell culture system. This work represents a pioneering step toward establishing ECM-based hydrogels as a promising biomaterial that recreates key features of the renal microenvironment and advancing progress toward therapies that could one day reduce dependence on dialysis and alleviate the burden on kidney transplantation.
Abstract Format
html
Abstract (Filipino)
Ang end-stage kidney disease (ESKD) ay nananatiling isang pangunahing pandaigdigang alalahanin sa kalusugan na nakasalalay lamang sa dalawang karaniwang paggamot, dialysis at kidney transplant. Ang mga hydrogel na gawa sa extracellular matrix (ECM) ay naghahandog ng isang makabago at malikhaing pamamaraan upang maunawaan ang pag-uugali ng mga selula at paglala ng sakit, na nagbibigay-daan sa pagbuo ng mga alternatibong pamamaraan ng paggamot sa ESKD. Sinuri ng pag-aaral na ito ang mga pisiko-kemikal na katangian ng mga hydrogel na gawa mula sa ECM ng mga bato ng baboy. Ang mga bato ng baboy ay matagumpay na na-decellularize gamit ang isang nobelang pamamaraan. Nagkaroon ng malinaw at makabuluhang pag-alis ng mga orihinal na selula at DNA (~ 97.6%). Nagkaroon rin ng makabuluhang pag-aalis ng ⍺-Gal (~95.8%), isang pangunahing xenoantigen na maaaring maging sanhi ng immune response. Ang resultang ECM mula sa na-decellularize na bato ng baboy ay ginamit upang gumawa ng mga hydrogel, na naka-crosslink gamit ang iba't-ibang konsentrasyon ng glutaraldehyde (GA). Batay sa resulta ng pisiko-kemikal na pagsusuri, ang 0.5 at 1.0% na GA-crosslinked hydrogels ay nagpakita ng hinahangad na mga katangian ng hydrogel. Nagpakita rin sila ng pare-parehong katatagan, may magaspang at butas-butas na anyo, at malawak na saklaw at iba’t-ibang sukat ng mga butas, na nagpapahiwatig ng kanilang potensyal na mapadali ang difusyon, paggalaw ng mga selula, at pag-usbong ng mga daluyan ng dugo. Nagpakita rin sila ng mas mataas na kapasidad ng paglapad, magkakaugnay na mga network, at pinanatili ang mahahalagang bahagi ng ECM, na nagbibigay-diin sa kanilang potensyal bilang angkop na biomaterial para sa mga aplikasyon sa tissue engineering, partikular bilang isang 3D cell culture system. Ang pag-aaral na ito ay kumakatawan sa isang makabagong hakbang tungo sa pagtatatag ng hydrogel, mula sa ECM ng mga bato ng baboy, bilang isang biomaterial na lumilikha ng mahahalagang kondisyon na matatagpuan sa totoong tisyu ng bato at nagpapabilis ng pag-buo ng mga pamamaraan sa paggamot, na balang-araw ay makakabawas sa pag-asa sa dialysis at magpapagaan sa pasanin na dulot ng kidney transplant.
Abstract Format
html
Language
English
Format
Electronic
Keywords
Kidneys—Diseases; Extracellular matrix; Glutaral; Colloids
Recommended Citation
Olaguer, L. T. (2025). Physicochemical characterization of glutaraldehyde-crosslinked hydrogels produced from the extracellular matrix (ECM) of decellularized porcine kidneys for tissue engineering applications. Retrieved from https://animorepository.dlsu.edu.ph/etdm_bio/31
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Embargo Period
12-11-2027
