Copper(II) detection using absorbance spectroscopy with anthocyanin

Date of Publication

5-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Premed Physics

Subject Categories

Physics

College

College of Science

Department/Unit

Physics

Thesis Advisor

Maria Cecilia D. Galvez

Defense Panel Chair

Edgar A. Vallar

Defense Panel Member

Wilfred V. Espulgar
Ofelia T. Rempillo

Abstract (English)

The presence of heavy metals in water samples could impose health risks for human consumption. With this, different techniques in water sample analysis through heavy metal detection have been developed. These techniques range from chemical analysis, spectroscopic methods, and chromatography. This study will determine the accuracy of heavy metal detection—particularly copper detection of ultraviolet-visible absorbance spectroscopy using anthocyanin tagger extracted from red cabbage (Brassica oleracea var. capitata f. rubra). Standard solutions of copper from 1.00 to 3.00 parts per million (ppm) with 0.50 ppm increment were used for the calibration curve to correlate the absorbance at 550 nm and the concentration of copper suspended in the sample. The calibration curve (R2 = 0.9677) was used to quantify the copper content of water samples collected. The water samples used in this study are collected from seven sampling sites: three stations in Pasig River, Awisan and Cooperative stations of river in Baguio, and Sta. Rosa and Taguig stations of Laguna Lake. Water sample copper concentrations were also determined using Atomic Absorption Spectroscopy (AAS) which served as the basis of the actual copper measurements. Copper concentration of four samples from absorbance spectroscopy measurements are not significantly different from the reports from copper quantification using AAS. This study shows that the technique used for copper quantification is not as accurate as other techniques despite the consistency of the reports of four water samples. This is because of huge percentage differences (highest at 91.35%) in each of the water samples. Also, there are other suspended matters which also absorb light at 550 nm that could affect the absorbance reports of copper concentration. To further improve this detection and quantification technique, sample treatment should be used to ensure that absorbance reports at around 550 nm are coming from anthocyanin-copper complex.

Abstract Format

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Abstract (Filipino)

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Abstract Format

html

Language

English

Format

Electronic

Keywords

Copper—Analysis; Water—Analysis

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Embargo Period

8-2-2025

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