Date of Publication
8-18-2024
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science Major in Physics
Subject Categories
Physics
College
College of Science
Department/Unit
Physics
Thesis Advisor
Wilfred V. Espulgar
Defense Panel Chair
Romeric F. Pobre,
Defense Panel Member
Gwen B. Castillon
Ramon Cruzpero
Glenn G. Oyong
Abstract/Summary
Small Cell Lung Cancer (SCLC) is characterized by an extremely rapid rate of cell proliferation, a high propensity for early metastasis, and a poorer prognosis. External beam radiotherapy (EBRT) is one of the most crucial therapeutic modalities for treating SCLC. While radiotherapy is considered effective, it is important to recognize that lung cells are highly susceptible to ionizing radiation. Additionally, radiotherapy is expensive, so finding cheaper alternatives is important to make it accessible to everyone, especially to those with lower incomes. Two agents can be used during this radiation therapy: radiosensitizers are agents that increase the sensitivity of tumor cells to radiation whereas radioprotective agents are agents used to reduce the effects of radiation to the healthy cells. This research was done to confirm whether the anticancer effect of citral, a biomolecule found in most citrus plants, was radioprotective or radiosensitive. The researchers used small cell lung cancer cell lines (H69PR) to check citral’s radiosensitizing properties whereas human dermal fibroblast neonatal cell lines (HDFn) was used to check citral’s radioprotective properties. Using Cs-137 as the radiation source, these cells were treated with citral and were exposed to radiation for 5, 10, and 15 minutes. Statistical analysis and ImageJ was used to compare the number of cell count remaining per varying time, it was concluded that without citral treatment, 100% of HDFn cells were killed compared to the cells with treatment which only killed 81.81%. On the other hand, more H69PR cell lines were killed with citral treatment (94.74%) compared to without citral treatment (96.43%). This research confirmed citral’s potential as a radioprotective agent rather than a radiosensitizer.
Abstract Format
html
Language
English
Format
Electronic
Keywords
Lungs; Cancer cells; Lungs—Cancer; Radiation—Physiological effect; Radiation-protective agents; Radiation-sensitizing agents
Recommended Citation
Rostrata, C. C., & Sambrana, M. M. (2024). Evaluation of citral as a radioprotective or radiosensitizing agent using Cs-137 radiation on H69PR small-cell lung cancer cell lines and HDFn human dermal fibroblast neonatal cell lines. Retrieved from https://animorepository.dlsu.edu.ph/etdb_physics/29
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Embargo Period
6-18-2026
