Date of Publication

4-2026

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Patricio Elvin L. Cantiller

Defense Panel Chair

Marigold O. Uba

Defense Panel Member

Gliceria B. Ramos
Michael B. Ples

Abstract (English)

Green tea is widely known for its many health benefits including reproductive health and embryonic development. The lesser-known variety of green tea, Hojicha, may presumably have similar and even higher phytochemical components. These can be attributed to certain variations in the process of producing Hojicha. The present study therefore aimed to screen the potential effects of Hojicha on the growth and development of the chick embryo (Gallus gallus domesticus) through angiogenic and morphometric analyses. While angiogenesis is a key indicator for embryonic growth, morphometric analysis can further assess the effect on the development of the embryo’s external anatomical features. Hojicha of varying doses i.e. low dose, medium dose, and high dose were administered to the chicken eggs on the 5th day of incubation and results were harvested on the 12th day of egg incubation. The following angiogenic parameters were included in the study: total vessel length, junction density, total number of junctions, vascular endpoints and vascular density. On the other hand, the morphometric parameters included length measurements of crown-to-rump, thigh, drumstick, shank, third toe, wing, bill, and neck. Results of the study after statistical analyses showed that the low dose Hojicha exhibited a significant increase in vascular endpoint formation, indicating potential enhancement of angiogenesis. Moreover, the shank and bill growth significantly responded to the medium and low doses of the Hojicha, respectively. These may be attributed to the presence and concentration of certain phytochemical compounds in Hojicha and thus, may further suggest the importance of optimal dosing and developmental timing when screening its biological effects on embryonic growth. The findings of this study may provide preliminary basis for application on avian biology research – possibly for nutraceutical usage to potentially aid mitigating egg quality variabilities.

Abstract Format

html

Abstract (Filipino)

Ang Green Tea ay kilala sa iba’t iba benepisyo ng pang kalusugan katulad ng kalusugang pang reproduktibo at pag develop ng embryo. Ang iba pang uri nito, ang Hojicha, ay pinaniniwalaang may parehong antas ng phytochemical components  at maaaring mag iba dahil sa dulot ng pagkakaiba ng proseso nito. Layunin ng pag aaral na ito ay upang suriin ang Hojicha  tungkol sa potensyal na epekto nito sa development ng chick embryo (Gallus gallus domesticus) sa pamamaraang angiogenic at morphometric analyses. Samakatuwid, Ang angiogenesis ay mahalaga upang ma bigyang pansin ang panlabas na anatomiko ng isang embryo. Pinakamababa, katamtaman at pinakamataas ang iba’t ibang doses ng hojicha ang ibinigay sa mga itlog sa ika-5 araw ng incubation, at ang mga resulta ay kinuha pagkasapit ng ika-12 na araw. Kabilang sa angiogenic parameters ay ang total vessel length, junction density, total number of junctions, vascular endpoints and vascular density. Samantala, saklaw ng morphometric parameters ay ang length measurements of crown-to-rump, thigh, drumstick, shank, third toe, wing, bill, and neck. Batay statistikal na resulta, ang pinakamababang dose ay mayroong makabuluhang pagtaas  sa vascular endpoint formation, na nagpapahiwatig sa mas pinabuting angiogenesis. Ang growth ng shank at bill ay bahagyang nagbago sa katamtaman at mababang doses. Alinsunod dito, maiuugnay ang mga resulta sa konsentrasyon at taglay na phytochemical compounds sa hojicha na nag papakita ng kahalagahan sa wastong pagbibigay ng dosing at developmental timing kaugnay sa pananaliksik ng biyolohikal na epekto sa embryonic growth. Ang mga nakuhang datos ay maaaring magsilbing daan at batayan sa aplikasyon sa avian development research at posible sa nutraceutical usage upang makatulong sa pagbawas ng variability sa kalidad ng mga itlog. 

Abstract Format

html

Language

English

Format

Electronic

Keywords

Green tea; Blood-vessels—Growth; Chickens—Embryos

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Embargo Period

4-17-2028

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Available for download on Monday, April 17, 2028

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