Date of Publication
2025
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology major in Medical Biology
Subject Categories
Biology
College
College of Science
Department/Unit
Biology
Thesis Advisor
Gliceria B. Ramos
Defense Panel Member
Patricio Elvin L. Cantiller
John Paul S. Domingo
Abstract (English)
Agaricus bisporus (white button mushroom) is a widely cultivated edible mushroom known for its nutritional value and medicinal properties. It contains various bioactive compounds with reported anti-inflammatory, antioxidant, antimicrobial, and anticancer effects. Recent studies have shown its potential role in modulating physiological processes, including angiogenesis. Given its therapeutic benefits, this study aimed to evaluate the effects of A. bisporus extract on angiogenesis using the duck chorioallantoic membrane (CAM) assay. Eighty-seven (87) one-day-fertilized duck eggs were divided into five groups. Group 1 served as the baseline control, providing a reference for normal and untreated development. Groups 2 to 5 received treatments on the 8th day of embryonic development: Group 2 (natural control) received 0.9% NaCl, while Groups 3 to 5 received low (20 μL), median (50 μL), and high (100 μL) doses of A. bisporus extract, respectively. Eggs were either treated with or without gel sponges for image and histological analysis. Results showed that the number of nodes and junctions in the CAM significantly differed between groups, with the medium-dose group showing the highest mean rank, suggesting enhanced angiogenesis at this given dose. In contrast, the low-dose group exhibited the lowest mean rank. A significant difference was observed between the low- and medium-dose groups, indicating that the medium dose represents the threshold at which A. bisporus begins to exert pro-angiogenic activity. Histological analysis, utilizing the gel foam, was inconclusive due to low sample viability, largely attributed to the gel sponge setup, which resulted in reduced embryo survival across all groups. Notably, the group without a gel sponge maintained 100% viability. Despite this, comparison of the high-dose and control groups revealed fewer and less branched vessels, with wider lumens and reduced vascular prominence in the high-dose group, suggesting possible anti-angiogenic effects at higher concentrations. These findings highlight the presence of a threshold concentration at the medium dose and the limitations posed by the gel sponge method for histological evaluation.
Abstract Format
html
Abstract (Filipino)
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Abstract Format
html
Language
English
Format
Electronic
Keywords
Cultivated mushroom; Blood-vessels—Growth; Mallard
Recommended Citation
Bendaña, C. D., Imperio, M. L., Navarro, K. M., & San Diego, S. A. (2025). Screening for the influence of agaricus bisporus (White button mushroom) on anas platyrhynchos domesticus L. (Philippine mallard duck) angiogenesis and embryonic development. Retrieved from https://animorepository.dlsu.edu.ph/etdb_bio/136
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Embargo Period
8-13-2026
