Date of Publication

8-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Mariquit M. De Los Reyes

Defense Panel Chair

John Martin S. Mondragon

Abstract (English)

The study explored the potential of chicken gizzard lining as a sustainable source of extracellular matrix (ECM), such as collagen, for potential tissue engineering applications. Decellularization removes cellular components while preserving extracellular matrix (ECM) integrity, enabling the production of bioscaffolds that support cell adhesion, proliferation, and differentiation. Though porcine and bovine tissues are commonly studied, chicken gizzard lining remains underexplored despite similar structural features. A sonicationassisted decellularization protocol using sodium dodecyl sulfate (SDS) in phosphate-buffered saline (PBS) was applied at varying time points (3, 6, 9 hr) and sonication power levels (0, 60, 180 W). Decellularization effectiveness and ECM preservation were evaluated through histological staining (H&E), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Histological scoring favored the 180 W 9 hr treatment for ECM preservation. However, the Kruskal-Wallis test showed no significant differences for Criterion A (Loss of Cells) and Criterion B (Preservation of ECM Fibers), and a significant difference only in Criterion C (ECM Architecture) with a p-value of 0.013. Dunn’s post-hoc test confirmed that 180 W 6 hr preserved ECM structure significantly better than 60 W 9 hr, affirming that higher sonication power leads to significantly more effective ECM architecture preservation. SEM analysis revealed porosity (~50 µm) sufficient for nutrient diffusion and cell penetration, while FTIR confirmed the presence of key collagen markers (amide I, II, III, and A) despite some structural changes. Further research should refine treatment parameters and assess in vivo biocompatibility and functionality. These findings highlight chicken gizzard lining as a promising, low-cost source of extracellular matrix (ECM). Overall, this work contributes to the growing field of regenerative medicine by introducing chicken gizzard lining as a novel, cost-effective biomaterial alternative for ECM-based scaffolds.

Abstract Format

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Abstract (Filipino)

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Abstract Format

html

Language

English

Format

Electronic

Keywords

Extracellular matrix; Tissue engineering

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Embargo Period

8-16-2026

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Available for download on Sunday, August 16, 2026

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