Infection rate, histopathology, and identification of Sarcocystis SPP. infection in slaughtered Philippine water buffaloes collected from the veterinary inspection board, Tondo, Manila
Date of Publication
1999
Document Type
Master's Thesis
Degree Name
Master of Science in Biology
Subject Categories
Animal Experimentation and Research | Other Immunology and Infectious Disease | Parasitology
College
College of Science
Department/Unit
Biology
Thesis Adviser
Dr. Florencia G. Claveria
Defense Panel Chair
Abelardo Geronimo
Defense Panel Member
Salvador Escueta, Jr.
Roima Lim
Abstract/Summary
Sarcocystis infection was detected in 83.33 percent of the 120 carcasses of Philippine water buffaloes surveyed in the Veterinary Inspection Board, Tondo, Manila. Macroscopic sarcocysts and two forms of microscopic sarcocysts, the spindle-shaped or fusiform cysts and the globular to oval cysts were noted. Microscopic sarcocysts occurring alone were the dominant form in all muscle tissue types compared to single infection with macroscopic sarcocysts or as mixed infections. Ultrastructural analysis of macroscopic and microscopic sarcocysts and their cyst wall revealed two distinct species of Sarcocystis infecting Philippine water buffaloes. These are the macroscopic species, Sarcocystis fusiformis with Type 23 cyst wall possessing highly-dendritic cauliflower-like projections with annulated microfilaments and numerous electron-dense granules and the microscopic species, Sarcocystis levinei (Dissanaike and Kan, 1978) Huong, Dubey and Uggla, 1997 exhibiting a Type 7 cyst wall with minute hair-like protrusions with expanded or dome-shaped base, an intermediate finger-like, and distal tapering segments which at some points join to form conical tufts. This report represents the first documented study of the occurrence of S. levinei in Philippine water buffaloes supported with ultrastructural analysis. Histopathological changes such as displacement of surrounding muscle tissue and that of the host sacolemma with accompanying tissue necrosis were apparent in muscle tissues infected with both macroscopic and microscopic sarcocysts. Degeneration of myofibrils and mitochondria were evident.
Abstract Format
html
Language
English
Format
Accession Number
TG02865
Shelf Location
Archives, The Learning Commons, 12F Henry Sy Sr. Hall
Physical Description
46 leaves
Keywords
Water buffalo -- Infections; Cattle -- Diseases; Cattle -- Parasites; Sarcocystosis; Protozoan diseases
Recommended Citation
Cruz, M. P. (1999). Infection rate, histopathology, and identification of Sarcocystis SPP. infection in slaughtered Philippine water buffaloes collected from the veterinary inspection board, Tondo, Manila. Retrieved from https://animorepository.dlsu.edu.ph/etd_masteral/1970