Antimicrobial susceptibility of enterobacteriaceae from street food sold in the vicinity of selected LRT-1 stations

Date of Publication

2018

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Marigold O. Uba

Abstract/Summary

Street food is one of the most sought-after inexpensive meal in the Philippines as it has become a fundamental source of sustenance for the general public. It is enjoyed by many because of its popular flavor, affordability, availability, and convenience. However, its reputation is preceded and stigmatized as unhealthy since bacterial contamination is often associated with it due to poor and improper handling and preparation practices. In the previous study of Baja and Salandanan (2017), a total of 71 Enterobacteriaceae isolates were collected from street food sold in the vicinity of selected LRT-1 stations (Doroteo Jose, Blumentrit, Monumento, and Balintawak). Out of 71 isolates, 49 were not identified and screened for antimicrobial sensitivity by Baja and Salandanan (2017), and therefore were utilized and tested in this study. However, only 48 were identified using biochemical tests as isolate S3C12 didn’t exhibit growth when retrieved from the stock culture. Biochemical test results showed that 35.4% (17 out of 48) were Enterobacter, 2.1% (1 out of 48) was Klebsiella, 2.1% (1 out of 48) was identified as Shigella while 60.4% (29 out of 48) of the isolates were not identified due to inconsistent and conflicting results. From the biochemical test results and colonial morphological identification, 26 representative isolates were subjected to antibiotic disc diffusion assay based on distinguishing characteristics. For the antibiotic disc-diffusion assay, 4 out of 26 (15%) isolates were considered multidrug resistant (MDR) after expressing resistance to more than three (3) antibiotics. Isolates which showed resistance to any of the Third Generation Cephalosporins (specifically ceftazidime) and any of the Carbapenems were subjected to confirmatory tests for Extended-Spectrum β-lactamase (ESBL) detection and Carbapenemase detection, respectively. For the confirmatory tests, 0 out of 2 (0%) isolates tested positive for ESBL production while 3 out of 4 (75%) isolates tested positive for the Carbapenamase production

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU017618

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer disc ; 4 3/4 in.

Keywords

Street food--Philippies; Enterobacteriaceae

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