Effect of various salts on the efficiency of transformation in Escherichia coli strain HB 101 with pGEM-4Z plasmid

Date of Publication

1992

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Abstract/Summary

Escherichia coli HB101 cells were treated with 50 mM and 100 mM of CaC12, MnC12 and CoC12 to see the effectivity and efficiency of the salts in transforming the cells into competent recipients for transformation with the plasmid pGEM4. Results showed the absence of transformation in the cells treated with the above salts.

Abstract Format

html

Language

English

Format

Print

Accession Number

TU05626

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

55 leaves ; Computer print-out.

Keywords

Salts--Physiological effect; Plasmids; Bacterial transformation

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