Identification of an exogenic metabolite and extraction of Polysaccharides from mushrooms
Date of Publication
2012
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biochemistry
College
College of Science
Department/Unit
Chemistry
Thesis Adviser
Anamy Maria C. Paano
Defense Panel Chair
Marissa G. Noel
Defense Panel Member
Emmanuel V. Garcia
Abstract/Summary
Two mushroom species were studied to identify possible biologically active compounds. The dichloromethane (DCM) extract of an unidentified mushroom (JD) from Bontoc was subjected to column chromatography using n-hexane, DCM, and Ethyl acetate (EtOAc) or combinations thereof. One fraction (JDD-16) was re-chromatographed and fractions of similar thin layer chromatogram (TLC) profiles were pooled together. The pooled fraction (JDD-116-38) was analyzed using spectroscopic techniques. Proton Nuclear Magnetic Resonance (1H NMR) and Gas Chromatography-- Mass Spectrometry (GC-MS). Fraction JDD-16-38 was identified as triethyl phosphate (TEP) with some unidentified impurities. The mushroom Lentinus sajor-caju was extracted with cold and hot water (LSC and LSH extracts). Precipitation was allowed to take place by addition of ethanol to the cold and hot water extract affording 4 fractions, the ethanol solubles (LSCS and LSHS) and the insolubles (LSCI and LSHI). The four extracts were analyzed by analytical reversed phase High Performance Liquid chromatography (HPLC). The chromatogram of the four extracts appeared to have similar constituents. Further analysis of these components will be done by LC-MS analysis and other chemical tests in a follow up study. The water extracts will also be submitted for bioactivity testing.
Abstract Format
html
Language
English
Format
Accession Number
TU17502
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
x, 38 leaves : illustrations (some color) ; 28 cm.
Keywords
Mushrooms; Polysaccharides
Recommended Citation
Ocampo, J. R., & San Diego, D. D. (2012). Identification of an exogenic metabolite and extraction of Polysaccharides from mushrooms. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/8483
