Bacterial analysis of air sample from the nursery ward of a tertiary hospital in Alabang, Muntinlupa City

Date of Publication

2014

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Esperanza C. Cabrera

Abstract/Summary

Air sample was collected from the nursery ward of tertiary government hospital in Alabang, Muntinlupa City. Sampling was conducted using the Andersen Staplex Air Sampler in July 2013. The bacterial load was found to be 90 CFU/ m3 of air, calculated from flow rate of 2.35 m3/hour and sampling period of 20 minutes. Polymerase chain reaction using 16s rDNA universal primers produced 1.5 kb amplicons in 66 isolates. PCR amplicons were sequenced using the forward and reverse primers, and were identified using the Basic Local Alignment Search Tool of The National Center for Biotechnology Information. Results showed the presence of 14 genera belonging to 24 different species of bacteria. The 35 isolatesor 53% were identified to belong to the genus Staphylococcus. The eight species of Staphylococcusisolated in the study have been reported to be opportunistic and known to cause infections.Pseudomonas(5 isolates) and Acinetobacter(4 isolates) are also known to be associated with healthcare-acquired infections. The study shows that it is imperative for the hospital administration to reassess the infection control program and be very strict in implementing aseptic procedures like proper handling of equipment, hand washing, hygiene, etc.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU022242

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer disc ; 4 3/4 in.

Keywords

Air—Microbiology; Nurseries—Microbiology

Embargo Period

5-4-2021

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