Polymerase chain reaction based detection of 1-aminocyclopropane-1-carboxylate deaminase gene in selected strains of pink pigmented facultative methylotrophic bacteria

Date of Publication

2009

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Bacteria | Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Anthony C. Lee

Abstract/Summary

Fifty-seven (57) PPFM bacterial isolates deposited at the DLSU Microbiology Laboratory were screened for the presence of ACC deaminase gene. Primers were designed using reported gene sequences from several plant growth promoting bacteria in the National Center for Biotechnological Information (NCBI). Four out of the fifty seven bacterial isolates yielded amplicons corresponding to the expected size of the target gene fragments. The root elongation assay revealed that there was a significant difference in the length of the roots of Vigna radiata (mungbean) grown with bacterial isolates Methylobacterium sp. 14a, Methylobacterium sp. C18, and Methylobacterium sp. C20 compared to the blank and negative control groups. Methylobacterium oryzae CBMB20, the strain obtained from the Korean Agriculture Culture Collection, although did not yield amplicon corresponding to the ACC deaminase gene using the designed primer, it however, showed a significant increase in mungbean root length.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU019031

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer disc ; 4 3/4 in.

Keywords

Deamination; Methylobacterium

Embargo Period

4-16-2021

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