Identification and morphological characterization of fungal endophytes isolated from healthy malunggay (Moringa oleifera) leaves

Date of Publication

2009

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Gliceria Ramos

Abstract/Summary

The study was conducted primarily to isolate, characterize, identify and classify endophytic fungi associated with leaves of Moringa oleifera (Malunggay). The samples were collected from San Pablo City, Laguna. Endophytic fungi were isolated using direct plating method. These fungi were cultivated in Sabouraud dextrose agar and grouped based on colonial morphology and microscopic features. A total of 6 morphologically distinct groups were identified as Aspergillus sp., Chrysosporium sp., Fusarium sp., Geotrichum sp., Rhizopus sp., and Trichoderma sp. using Malloch dichotomous and photographic keys. Geotrichum candidum has the highest percentage occurrence of 50.02%. It is followed by Chrysosporium sp., Mucor sp., Trichoderma sp., and Fusarium sp. with 21.18%, 12.71%, 6.77%, and 5.93% respectively. Aspergillus sp. has the lowest percentage occurrence of 3.39%. Endophyte Incidence was 65.56% with 118/180 M. oleifera leaflets harboring fungal endophyte symbionts.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU019089

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer optical disc ; 4 3/4 in.

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