Agarose gel electrophoresis patterns of human sera in various disease states
Date of Publication
1995
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Abstract/Summary
Serum contains proteins such as albumin and globulins that can be separated into different fractions by agarose gel electrophoresis. The resulting pattern, especially that of samples extracted from patients of a particular disease, is important because it could be used as a gauge to monitor and diagnose that disease.43 samples were obtained and divided as follows: 9 with liver diseases, 4 with heart diseases, 6 with pulmonary diseases, 1 with a neoplastic lung disease, 6 with endocrine diseases, 5 with renal diseases, 1 with tumor, 9 with blood disorders, 1 with disease of the joints and 1 from a drug addict. Data were analyzed by comparing the samples to a normal serum pattern and from results of previous studies.Results showed that proteins were altered as caused by the nature of the disease and were manifested electrophoretically by the difference between each bands of the protein pattern of the sample and the normal serum. The most striking difference from a normal serum sample were seen in multiple myeloma patients while patients with hepatitis B, pulmonary tuberculosis stage 3 with massive hemoptysis, arteriosclerotic heart disease and the drug addict showed no electrophoretic difference from the normal.
Abstract Format
html
Language
English
Format
Accession Number
TU07312
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
52 leaves
Keywords
Gel electrophoresis; Separation (Technology); Blood plasma; Blood--Diseases--Diagnosis
Recommended Citation
Espiritu, G. V. (1995). Agarose gel electrophoresis patterns of human sera in various disease states. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/1335