Prevalence of nonsocomial methicillin-resistant staphylococcus aureus (MRSA) from cases of a tertiary hospital in Metro Manila and the occurrence of pantonvalentine leukocidin gene

Date of Publication

2010

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Adviser

Esperanza Cabrera

Abstract/Summary

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as one of the most frequent causes of nosocomial infection in hospitals worldwide. Hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) poses a great threat to the community. It is resistant to all β- lactam antibiotics and many strains have been reported to also be resistant to other classes of antimicrobials. The present study determined the antimicrobial susceptibility patterns of S. aureus from patients with nosocomial infections in a tertiary hospital in Metro Manila using the disc diffusion assay. It also determined the resistance of the isolates to methicillin using cefoxitin and oxacillin discs, and confirmed using PCR to detect the mecA gene. The mecA gene was detected in strains phenotypically identified to be methicillin resistant. Of the 143 nosocomial S. aureus, 66 or 46.2% were confirmed to be MRSA. These isolates were found to be negative for the luks-lukf Panton-Valentine leukocidin genes. Resistance to penicillin, erythromycin, azithromycin, trimethoprim sulfamethoxazole, gentamicin, ciprofloxacin, gatifloxacin, tetracycline, chloramphenicol, vancomycin and linezolid were shown by 60.1%, 51.7%, 51.7%, 39.2%, 37.8%, 37.1%, 36.4%, 14.0%, 2.80%, 1.40% and 0.700% of the isolates, respectively. It is worthy to note that two of the isolates were found to be resistant to vancomycin, the drug choice for treatment of MRSA infections.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTU019216

Shelf Location

Archives, The Learning Commons, 12F, Henry Sy Sr. Hall

Physical Description

1 computer optical disk ; 4 3/4 in.

Keywords

Staphylococcus aureus infections; Staphylococcal infections; Methicillin resistance.

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