Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
Date of Publication
1992
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology
College
College of Science
Department/Unit
Biology
Abstract/Summary
Under controlled laboratory conditions of water temperature (25-26 centigrade) and salinity (30-34 ppt), the developmental time table of the sea urchin, Diadema setosum was established. Gametes were obtained via the chemical induction techniques by Kobayashi (1971). The sperm and eggs were mixed and allowed to develop in culture beakers containing filtered aerated sea water. The developing embryos were observed microscopically (2150X) at different time points within the 48-hour culture period.The study showed that fertilization was attained 3-4 minutes after the sperms were added to the eggs. The 2-cell stage was observed 60-70 minutes, blastula stage recorded after 300 minutes and mid-gastrula 600 minutes after the sperms were added to the eggs. The 4-armed pluteal larvae stage was the highest developmental stage attained within 1860-1920 minutes.
Abstract Format
html
Language
English
Format
Accession Number
TU05639
Shelf Location
Archives, The Learning Commons, 12F, Henry Sy Sr. Hall
Physical Description
54 leaves
Keywords
Sea-urchins; Fertilization (Biology); Embryology; Experimental
Recommended Citation
Perez, M. H. (1992). Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas. Retrieved from https://animorepository.dlsu.edu.ph/etd_bachelors/1100
