Phenotypic characterization of pink pigmented facultative methylothrophic (PPFM) bacteria from various sources

Authors

Thaddeus M. Carvajal, De La Salle University, Manila
M.A. M. Dolleton, De La Salle University, Manila
O. A. Gagui, De La Salle University, Manila
E. C. Peralta, De La Salle University, Manila
M.C. D. Valera, De La Salle University, Manila
Anthony C. Lee, De La Salle University, Manila

College

College of Science

Department/Unit

Biology

Document Type

Archival Material/Manuscript

Publication Date

2007

Abstract

A total of eighteen (18) pink-pigmented facultative methylotrophic (PPFM) bacteria from various habitats were isolated and described. A mineral salt agar medium with 0.5% methanol was used as the selective medium for the isolation. Pure cultures were obtained after re-streaking in selective as well as non-selective media. The obtained isolated were gram-negative rods with some unstained cyst like structures. The cyst like structures stained black after polyß-hydroxybutyrate staining with Sudan Black. All of the isolates grew in brain heart infusion agar, nutrient agar, glycerol peptone agar, and eosin methylene blue agar but not in MacConkey agar. Optimum incubation temperature for the isolates varied from room temperature (25⁰C) and 37⁰C. The isolates exhibited varied biological activities based on the different biochemical tests performed.

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Recommended Citation

Carvajal, T. M., Dolleton, M. M., Gagui, O. A., Peralta, E. C., Valera, M. D., & Lee, A. C. (2007). Phenotypic characterization of pink pigmented facultative methylothrophic (PPFM) bacteria from various sources. Retrieved from https://animorepository.dlsu.edu.ph/faculty_research/7774

Disciplines

Biology

Keywords

Methylotrophic bacteria

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