Effect of high temperature on protein expression in strawberry plants
College
College of Science
Department/Unit
Biology
Document Type
Article
Source Title
Biologia Plantarum
Volume
48
Issue
1
First Page
73
Last Page
79
Publication Date
2004
Abstract
Strawberry plants (Fragaria×ananassa Duch.) cvs. Nyoho and Toyonoka were exposed to temperatures of 20, 33, and 42 °C for 4 h, and protein patterns in leaves and flowers was analyzed by 2-dimensional polyacrylamide gel electrophoresis and immunoblotting. In leaves and flowers of both cultivars, the content of most proteins decreased, but a few new proteins appeared in response to heat stress. These heat shock proteins (Hsps) were detected in the range of 19 – 29 kDa in leaves, and 16 – 26 kDa in flowers. The intensity of a 43 kDa protein spot increased in response to heat stress in Nyoho flowers, but not in Toyonoka flowers. The peaHsp17.7 antibody recognized one band at approximately 26 kDa in leaves, and two bands at approximately 16 and 17 kDa in flowers of both cultivars. These results show that the effects of heat stress on Hsp synthesis in strawberry plants differ between plant organs and between cultivars.
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Recommended Citation
Ledesma, N. A., Kawabata, S., & Sugiyama, N. (2004). Effect of high temperature on protein expression in strawberry plants. Biologia Plantarum, 48 (1), 73-79. Retrieved from https://animorepository.dlsu.edu.ph/faculty_research/7766
Disciplines
Biology
Keywords
Plant proteins—Effect of temperature on; Strawberries—Effect of temperature on
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