Cytotoxic compounds from Dysoxylum gaudichaudianum (A. Juss.) Miq.

Authors

Mariquit M. De Los Reyes, De Salle University Science and Technology
Glenn G. Oyong, De La Salle University, Manila
Vincent Antonio S. Ng, De La Salle University, Manila
Chien Chang Shen, National Research Institute of Chinese Medicine Taiwan
Consolacion Y. Ragasa, De La Salle University, Manila

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

International Journal of Pharmacognosy and Phytochemical Research

Volume

8

Issue

4

First Page

663

Last Page

667

Publication Date

4-1-2016

Abstract

© 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. Squalene (1), polyprenol (2), triglycerides (3), and β-sitosterol (4), isolated from the dichloromethane extract of the leaves of Dysoxylum gaudichaudianum, were evaluated for their anti-proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast neonatal (HDFn) using the in vitro PrestoBlue® cell viability assay. The HT-29 cell line was most susceptible to the compounds tested. Squalene (1) and polyprenol (2) were most cytotoxic against HT-29 (IC50 of 0.84 and 1.059 μg/mL, respectively), followed by MCF-7 (IC50 of 4.404 and 3.786 μg/mL, respectively), and HCT-116 (IC50 of 7.859 and 9.258 μg/mL, respectively). Triglycerides (3) was highly anti-proliferative against HT-29 cells (IC50 of 2.738 μg/mL) and moderately inhibitory against HCT-116 and MCF-7 cells (IC50 of 13.05 and 16.12 μg/mL, respectively). β-Sitosterol (4) was most cytotoxic against HT-29 and MCF-7 (IC50 of 1.41 and 2.277 μg/mL, respectively) and moderately cytotoxic against HCT-116 (IC50 of 11.63 μg/mL). Comparing the cytotoxic effects of these compounds against the three cancer cell lines, 1-4 were most cytotoxic against HT-29. Compounds 1, 2 and 4 were less inhibitory to MCF-7 and least inhibitory to HCT-116. Comparing the effects of 1-4 on the two colon cancer cell lines, their IC50 values against HT-29 were lower than those of HCT-116. Compounds 1-4 showed moderate cytotoxicity against HDFn cells (11.18 to 17.72 μg/mL) and were less cytotoxic to the normal cells than the cancer cells.

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Recommended Citation

De Los Reyes, M. M., Oyong, G. G., Ng, V. S., Shen, C., & Ragasa, C. Y. (2016). Cytotoxic compounds from Dysoxylum gaudichaudianum (A. Juss.) Miq.. International Journal of Pharmacognosy and Phytochemical Research, 8 (4), 663-667. Retrieved from https://animorepository.dlsu.edu.ph/faculty_research/734

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