DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods
College
College of Science
Department/Unit
Biology
Document Type
Article
Source Title
Physiological and Molecular Plant Pathology
Volume
107
First Page
14
Last Page
20
Publication Date
8-1-2019
Abstract
© 2019 Elsevier Ltd A nanobiosensor was developed for the electrochemical detection of Phytophthora palmivora, a notorious pathogen of cacao causing severe crop loss. Sandwich hybrids between two oligonucleotide probes and the genomic DNA of P. palmivora were prepared and electrochemically detected. The oligonucleotide probes were designed based on the ITS sequence of the P. palmivora field isolates. The detection of sandwich hybrids with P. palmivora genomic DNA and the selectivity of the nanobiosensor towards it compared to other cacao-associated fungal pathogens were demonstrated. The detection limit was determined to be at 0.30 ng DNA μL−1. Detection of P. palmivora DNA on cacao samples was also demonstrated. The result of the preliminary analysis on cacao pod samples shows the potential of the developed nanobiosensor for reliable and more cost-effective analysis of field samples.
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Digitial Object Identifier (DOI)
10.1016/j.pmpp.2019.04.004
Recommended Citation
Franco, A. D., Merca, F. E., Rodriguez, M. S., Balidion, J. F., Migo, V. P., Amalin, D. M., Alocilja, E. C., & Fernando, L. M. (2019). DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods. Physiological and Molecular Plant Pathology, 107, 14-20. https://doi.org/10.1016/j.pmpp.2019.04.004
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