Radioprotection of intestinal crypt cells by cox-inhibitors

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

Philippine Journal of Science

Volume

135

Issue

2

First Page

73

Last Page

81

Publication Date

12-2006

Abstract

The regulation of tissue homeostasis in the gastrointestinal epithelium after epithelial injury focuses on the prostaglandins (PGs) as its major mediators. The two cyclooxygenase isoforms, Cox-1 and Cox-2, catalyze synthesis of PGs. Cox-1 is the predominant cyclooxygenase isoform found in the normal intestine. In contrast, Cox-2 is present at low levels in normal intestine but is elevated at sites of inflammation, and in adenomas and carcinomas. To study the effects of various commercially-available cox-inhibitors (Ketorolac: Cox-1 selective; Celecoxib: Cox-2 selective; and Indocid: Cox-1/2 non-selective), we determine mouse crypt epithelial cell fate after genotoxic injury with whole-body gamma-ray exposure at 15 Gy. Intestinal tissues of mice treated with Cox-2 inhibitors that showed invariable apoptotic event, however, have increased occurrence of regenerating cells. Our results suggest a potential application of Cox-2 selective inhibitors as radioprotective agent for normal cells after radiotherapy.

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Disciplines

Biology | Cell and Developmental Biology

Keywords

Cyclooxygenase 2—Inhibitors; Epithelial cells; Prostaglandins; Apoptosis

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