Bioethanol production from microalgae Chlorella sorokiniana via simultaneous saccharification and fermentation

College

Gokongwei College of Engineering

Department/Unit

Chemical Engineering

Document Type

Conference Proceeding

Source Title

IOP Conference Series: Materials Science and Engineering

Volume

778

Issue

1

Publication Date

4-30-2020

Abstract

This study aimed to investigate the performance of a microalgal feedstock Chlorella sorokiniana in bioethanol production through simultaneous saccharification and fermentation (SSF). The major limiting factor of SSF is the compensation between optimum hydrolysis and fermentation parameters. Using a central composite design (CCD), three parameters were optimized: biomass concentration, temperature, and pH. For each run, the enzyme mixture (cellulase and amylase) was fixed at 25 g/L, fermentation time at 72 hrs, and inoculum size of Saccharomyces cerevisiae at 20% (v/v). The freeze-dried biomass is simultaneously mixed with the enzyme mixture and the inoculum and placed inside an incubator shaker. The microalgal biomass is found to contain 58.78% total reducing sugars (TRS). Highest ethanol yield obtained is 0.504 g ethanol/g glucose. Using CCD helps navigate the design space and bioprocess. © 2020 IOP Publishing Ltd.

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Digitial Object Identifier (DOI)

10.1088/1757-899X/778/1/012039

Disciplines

Chemical Engineering

Keywords

Chlorella sorokiniana; Biomass energy; Ethanol as fuel

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