Sonication-assisted perfusion decellularization of whole porcine kidney

College

Gokongwei College of Engineering

Department/Unit

Chemical Engineering

Document Type

Article

Source Title

International Journal of Biology and Biomedical Engineering

Volume

13

First Page

78

Last Page

81

Publication Date

1-1-2019

Abstract

Bioengineering of kidneys is a potential treatment option in addressing common problems such as incompatibility and shortage of donor organs. The first step in the kidney bioengineering process involves perfusion decellularization wherein the use of chemicals is considered the most preferred preparation method to date. However, the use of chemicals alone requires long treatment time and excessive chemical usage hence, in this study perfusion decellularization was enhanced by sonication treatment at varying sonication power (150, 200 and 250 W). Scaffolds produced were evaluated for cell removal and preservation of structural integrity. Results revealed that decellularization with sonication using 150 W, 200 W and 250 W required a treatment time of 24 h, 16 h, and 12 h respectively compared to the 28 h treatment time of decellularization without sonication. Cells were almost and completely removed as indicated by histological analysis. Meanwhile, preservation of renal structures such as glomerulus, tubules, and blood vessels were observed except for the kidney scaffolds produced from decellularization with 250 W sonication where minimal disruption of the glomerular basement membranes and thinning of blood vessels were observed. Overall, decellularization with 200 W of sonication power resulted in an acellular renal ECM scaffold and preserved ECM structure. It can also be concluded that the higher sonication power used, the shorter is the decellularization time needed to prepare a kidney scaffold thus reducing the amount of chemicals used. © 2019 North Atlantic University Union NAUN. All rights reserved.

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Disciplines

Biochemical and Biomolecular Engineering | Chemical Engineering

Keywords

Extracellular matrix; Kidneys—Diseases; Perfusion (Physiology); Sonication

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