Vacuum fermentation integrated with separation process for ethanol production

College

Gokongwei College of Engineering

Department/Unit

Chemical Engineering

Document Type

Article

Source Title

Biochemical Engineering Journal

Volume

55

Issue

3

First Page

208

Last Page

214

Publication Date

8-15-2011

Abstract

In this study, continuous fermentation integrated with separation process at atmospheric and vacuum pressures are investigated. An initial glucose concentration of 200gL-1 was used to produce yeast cells under batch operating mode. After a period of 18-20h, a medium of 350gL-1 glucose concentration was fed continuously to the fermentation-separation column. During the continuous experiments, fermented broth was recirculated back to the fermentation-separation column. At atmospheric pressure, the dry mass concentration decreased rapidly and remained at 2.4gL-1 after long period, while glucose concentration still remained at 240gL-1. On the other hand, at vacuum pressure, a quasi-steady state condition was reached after 96h, corresponding to 4.2gL-1 of cell dry mass, 165gL-1 of glucose and 44.2gL-1 of ethanol levels in the fermentation broth and 33.2wt% of ethanol concentration at the outlet. These results indicate reduced product and substrate inhibition, suggesting that the integrated process could be used to replace the first bioreactor in a fermentation cascade system. A mathematical model was then developed and used to predict the kinetic parameters. The simulation results agreed well with experimental data. © 2011 Elsevier B.V.

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Digitial Object Identifier (DOI)

10.1016/j.bej.2011.05.001

Disciplines

Chemical Engineering

Keywords

Fermentation; Vacuum technology; Separation (Technology); Ethanol

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