Teratogenic effect of papaya (Carica papaya) seed extracts on the embryonic development of zebrafish (Danio rerio)

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

Advances in Environmental Biology

Volume

9

Issue

18

First Page

91

Last Page

96

Publication Date

8-1-2015

Abstract

With the teratogenic assay using D. rerio embryonic model, this present work established the toxic and teratogenic effects of extracts of the two C. papaya seed types, young and mature. Bioactive compositions were obtained through hot water extraction. Embryos at segmentation phase were exposed to the varying extract concentrations. Mortality, hatchability, and morphological malformations were determined. Based on the results, the incidences of mortality and most teratogenic effects were apparently concentration-dependent. Embryos exposed at 10% of young seed extract and 3% of mature seed extract significantly recorded 100% mortality after 48 hours of treatment exposure. Coagulation of embryo was the most notable toxic effect of both extracts. Hatchability of 0.1% and 0.3% treated embryos was significantly lower than that of the control embryos. No hatched was recorded in 1% or higher concentrations. Embryos at 0.1% (young seed) and 0.03% (mature seed) or higher extract concentrations showed delayed development. Tail malformation (bent tail or hook-like tail) is a fingerprint morphological endpoint of both C. papaya seed extracts. At 72 hour post treatment application (hpta), tail malformation was observed in embryos at 0.03% (for mature seed) and 0.1% (for young seed) or higher concentrations. Altogether, the extracts of the two C. papaya seed types affect the survival and embryonic development of D. rerio, however, mature seed extract is found more toxic than the young seed extract. © 2015 AENSI Publisher All rights reserved.

html

Keywords

Papaya—Seeds; Teratogenicity testing; Zebra danio—Embryos

Upload File

wf_no

This document is currently not available here.

Share

COinS