Effects of pulsed electromagnetic fields on breast cancer cell line MCF 7 using absorption spectroscopy

College

College of Science

Department/Unit

Physics

Document Type

Article

Source Title

Anticancer Research

Volume

37

Issue

7

First Page

3453

Last Page

3459

Publication Date

7-1-2017

Abstract

We present an analysis of the effects of pulsed electromagnetic fields (PEMF) with 3.3 MHz carrier frequency and modulated by audio resonant frequencies on the MCF-7 breast cancer cell line in vitro using absorption spectroscopy. This involves a fluorescence dye called PrestoBlue™ Cell Viability Reagent and a spectrophotometry to test the viability of MCF-7 breast cancer cells under different PEMF treatment conditions in terms of the cell absorption valueS. The DNA molecule of the MCF-7 breast cancer cells has an electric dipole property that renders it sensitive and reactive to applied electromagnetic fieldS. Resonant frequencies derived from four genes mutated in MCF-7 breast cancer cells [rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR), peroxisome proliferator-activated receptor (PPARG), Nijmegen breakage syndrome 1 (NBN) and checkpoint kinase 2 (CHEK2)] were applied in generating square pulsed electromagnetic waveS. Effects were monitored through measurement of absorption of the samples with PrestoBlue™, and the significance of the treatment was determined using the t-test. There was a significant effect on MCF-7 cells after treatment with PEMF at the resonant frequencies of the following genes for specific durations of exposure: RICTOR for 10 min, PPARG for 10 min, NBN for 15 min, and CHEK2 for 5 min.

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Digitial Object Identifier (DOI)

10.21873/anticanreS.11713

Disciplines

Physics

Keywords

Breast—Cancer; Electromagnetic fields; Mutation (Biology); Spectrum analysis

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