Effects of pulsed electromagnetic fields on breast cancer cell line MCF 7 using absorption spectroscopy
College
College of Science
Department/Unit
Physics
Document Type
Article
Source Title
Anticancer Research
Volume
37
Issue
7
First Page
3453
Last Page
3459
Publication Date
7-1-2017
Abstract
We present an analysis of the effects of pulsed electromagnetic fields (PEMF) with 3.3 MHz carrier frequency and modulated by audio resonant frequencies on the MCF-7 breast cancer cell line in vitro using absorption spectroscopy. This involves a fluorescence dye called PrestoBlue™ Cell Viability Reagent and a spectrophotometry to test the viability of MCF-7 breast cancer cells under different PEMF treatment conditions in terms of the cell absorption valueS. The DNA molecule of the MCF-7 breast cancer cells has an electric dipole property that renders it sensitive and reactive to applied electromagnetic fieldS. Resonant frequencies derived from four genes mutated in MCF-7 breast cancer cells [rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR), peroxisome proliferator-activated receptor (PPARG), Nijmegen breakage syndrome 1 (NBN) and checkpoint kinase 2 (CHEK2)] were applied in generating square pulsed electromagnetic waveS. Effects were monitored through measurement of absorption of the samples with PrestoBlue™, and the significance of the treatment was determined using the t-test. There was a significant effect on MCF-7 cells after treatment with PEMF at the resonant frequencies of the following genes for specific durations of exposure: RICTOR for 10 min, PPARG for 10 min, NBN for 15 min, and CHEK2 for 5 min.
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Digitial Object Identifier (DOI)
10.21873/anticanreS.11713
Recommended Citation
Alcantara, D. Z., Soliman, I. S., Pobre, R. F., & Naguib, R. G. (2017). Effects of pulsed electromagnetic fields on breast cancer cell line MCF 7 using absorption spectroscopy. Anticancer Research, 37 (7), 3453-3459. https://doi.org/10.21873/anticanreS.11713
Disciplines
Physics
Keywords
Breast—Cancer; Electromagnetic fields; Mutation (Biology); Spectrum analysis
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