Chemical characterization of Berberis vulgaris L. Var. asperma edible berries

College

College of Science

Department/Unit

Chemistry

Document Type

Article

Source Title

Malaysian Journal of Analytical Sciences

Volume

22

Issue

4

First Page

579

Last Page

585

Publication Date

1-1-2018

Abstract

Berberis vulgaris L. var. asperma edible fruits have been found to have activity against several microbes and to be cytotoxic in certain immortalized cancer cell lines. In this study, the berries were analyzed to find the possible metabolites that could be the curative agents. Evaluations using an LC-UV/MS technique established the presence of chlorogenic acid, rutin, and berberine in ethanolic extracts of B. vulgaris berries. The ethanolic sample was found to have 47.1 mg/kg of 3-(3,4-dihydroxycinnamoyl) quinic acid, 0.02 mg/kg of rutin and 0.36 mg/kg of berberine. The free radical scavenging activity of the mixture of polyphenolics exhibited an IC50 at 34.48 µg/mL. An assessment of zinc and iron concentration using atomic absorbance spectrophotometry (AAS) was determined to be 45.3 and 198.1 mg/kg, respectively. The investigation of the volatile constituents through solid phase microextraction (SPME) and by dichloromethane extraction was explored using a gas chromatograph equipped with a mass spectrometer (GC-EI-MS). SPME analyses showed the presence of palmitic acid (C16:0), vaccenic acid (C18:1 Δ 11 trans) and stearic acid (C18:0). Three saturated fatty acids, SFAs (C14:0, C16:0, and C18:0), two methyl esters of the SFAs (C14:0 and C16:0), and one polyunsaturated fatty acid, PUFA (C18:2 Δ 9,12 cis) were detected in the dichloromethane extracts of the berries. © 2018, Malaysian Society of Analytical Sciences. All rights reserved.

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Digitial Object Identifier (DOI)

10.17576/mjas-2018-2204-02

Disciplines

Chemistry

Keywords

Barberries--Analysis; Berberidaceae--Analysis

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