Multimeric TAT peptides are effective in vitro inhibitors of Staphylococcus saprophyticus
College
College of Science
Department/Unit
Chemistry
Document Type
Letter to the Editor
Source Title
Chemical Biology and Drug Design
Publication Date
1-1-2020
Abstract
© 2020 John Wiley & Sons Ltd TAT (48–60) is a tridecapeptide from the envelope protein of HIV that was previously shown to possess cell-penetrating properties and antibacterial activity, making it a potential drug delivery agent for anticancer drugs and as antibacterial compound. Previous reports indicated that dimerization enhances the desired bioactivity of TAT; hence, we sought to synthesize multimeric TAT peptides. Herein, we describe the effects of multimerization on the antibacterial activity and secondary structure of the peptide. Terminal modifications such as N-acetylation and C-amidation were employed in the design. TATp monomer, dimer, and tetramer were synthesized using solid-phase peptide synthesis, purified by reversed-phase HPLC, and then characterized by mass spectrometry. Multimerization of the peptide did not change the secondary structure conformation. The CD analysis revealed a polyproline-II conformation for all peptide designs. Thus, this study provides a method of increasing the biological activity of the peptide by multimerization while retaining the secondary conformation of its monomeric unit. Furthermore, the bacteria Staphylococcus saprophyticus was found to be susceptible to the dimer and tetramer, with MIC50 of 12.50 μm and <1.56 μm, respectively. This suggests a structure–activity relationship whereby the antibacterial activity increases with increase in valency.
html
Digitial Object Identifier (DOI)
10.1111/cbdd.13706
Recommended Citation
Chiongson, J., Sabido, E., Lin, K., Alea, G., Dalisay, D., Wu, S., & Saludes, J. (2020). Multimeric TAT peptides are effective in vitro inhibitors of Staphylococcus saprophyticus. Chemical Biology and Drug Design https://doi.org/10.1111/cbdd.13706
