High genetic diversity of Anaplasma marginale detected from Philippine cattle
College
College of Science
Department/Unit
Biology
Document Type
Article
Source Title
Journal of Veterinary Medical Science
Volume
76
Issue
7
First Page
1009
Last Page
1014
Publication Date
1-1-2014
Abstract
A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle. © 2014 The Japanese Society of Veterinary Science.
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Digitial Object Identifier (DOI)
10.1292/jvms.13-0405
Recommended Citation
Ybañez, A. P., Ybañez, R. D., Claveria, F. G., Flores, M. C., Xuenan, X., Yokoyama, N., & Inokuma, H. (2014). High genetic diversity of Anaplasma marginale detected from Philippine cattle. Journal of Veterinary Medical Science, 76 (7), 1009-1014. https://doi.org/10.1292/jvms.13-0405
Disciplines
Biology
Keywords
Anaplasma marginale; Cattle—Diseases--Philippines
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