High genetic diversity of Anaplasma marginale detected from Philippine cattle

College

College of Science

Department/Unit

Biology

Document Type

Article

Source Title

Journal of Veterinary Medical Science

Volume

76

Issue

7

First Page

1009

Last Page

1014

Publication Date

1-1-2014

Abstract

A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle. © 2014 The Japanese Society of Veterinary Science.

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Digitial Object Identifier (DOI)

10.1292/jvms.13-0405

Disciplines

Biology

Keywords

Anaplasma marginale; Cattle—Diseases--Philippines

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