Antioxidant, cytotoxicity, and antiophidian potential of Alstonia macrophylla bark

Authors

Maria Carmen S. Tan, De La Salle University, Manila
Mary Stephanie S. Carranza, De La Salle University, Manila
Virgilio C. Linis, De La Salle University, Manila
Raymond S. Malabed, De La Salle University, Manila
Glenn G. Oyong, Osaka University

College

College of Science

Department/Unit

Chemistry

Document Type

Article

Source Title

ACS Omega

Volume

4

Issue

5

First Page

9488

Last Page

9496

Publication Date

5-30-2019

Abstract

The objective of this research was to find the possible pharmacognosy of the bark of the Philippine Alstonia macrophylla Wall. ex G.Don (AM). Gas chromatographic-mass spectral (GC-EI-MS) characterization and energy dispersive X-ray spectroscopy (EDX) were performed to detect the bioactive constituents. EDX analysis of AM bark displayed a high content of potassium (3.26%) and calcium (2.96%). Eight constituents were detected in AM crude dichloromethane (DCM) extracts, which consisted of a long-chain unsaturated fatty acid (17:0) and fatty acid esters such as ethyl hexadecanoate and methyl hexadecanoate. Extraction of AM bark using methanol and dimethyl sulfoxide (MeOH/DMSO) solvents resulted in the identification of 17 constituents, principally alkaloids (alstonerine, 34.38%; strictamin, 5.23%; rauvomitin, 4.29%; and brucine, 3.66%) and triterpenoids (γ-sitosterol, 3.85%; lupeol, 3.00%; 24-methylenecycloartanol, 2.81%; campesterol, 2.71%; β-amyrin, 2.30%; and stigmasterol, 2.13%). MeOH/DMSO samples of AM were used in the selected bioassays. The samples exhibited efficient free radical scavenging activity (IC50 = 0.71 mg/mL) and were noncytotoxic to normal HDFn (IC50 > 100 μg/mL) and neoplastic THP-1 cell lines (IC50 = 67.22 μg/mL) while highly degenerative to MCF-7 (IC50 = 6.34 μg/mL), H69PR (IC50 = 7.05 μg/mL), and HT-29 (IC50 = 9.10 μg/mL). Most interestingly, the AM samples inhibited the northern Philippine Cobra's (Naja philippinensis Taylor) venom (IC50 = 297.27 ± 9.33 μg/mL) through a secretory phospholipase A2 assay.

html

Digitial Object Identifier (DOI)

10.1021/acsomega.9b00082

Recommended Citation

Tan, M. S., Carranza, M. S., Linis, V. C., Malabed, R. S., & Oyong, G. G. (2019). Antioxidant, cytotoxicity, and antiophidian potential of Alstonia macrophylla bark. ACS Omega, 4 (5), 9488-9496. https://doi.org/10.1021/acsomega.9b00082

Disciplines

Chemistry

Keywords

Alstonia—Therapeutic use—Philippines

Upload File

wf_yes