Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions
College
College of Science
Department/Unit
Biology
Document Type
Article
Source Title
Plant Biotechnology Journal
Volume
17
Issue
7
First Page
1289
Last Page
1301
Publication Date
7-1-2019
Abstract
In legumes, nitrogen (N) can be stored as ureide allantoin and transported by ureide permease (UPS) from nodules to leaves where it is catabolized to release ammonium and assimilation to amino acids. In non-leguminous plants especially rice, information on its roles in N metabolism is scarce. Here, we show that OsUPS1 is localized in plasma membranes and are highly expressed in vascular tissues of rice. We further evaluated an activation tagging rice overexpressing OsUPS1 (OsUPS1OX) under several N regimes. Under normal field conditions, panicles from OsUPS1OX plants (14 days after flowering (DAF)) showed significant allantoin accumulation. Under hydroponic system at the vegetative stage, plants were exposed to N-starvation and measured the ammonium in roots after resupplying with ammonium sulphate. OsUPS1OX plants displayed higher ammonium uptake in roots compared to wild type (WT). When grown under low-N soil supplemented with different N-concentrations, OsUPS1OX exhibited better growth at 50% N showing higher chlorophyll, tiller number and at least 20% increase in shoot and root biomass relative to WT. To further confirm the effects of regulating the expression of OsUPS1, we evaluated whole-body-overexpressing plants driven by the GOS2 promoter (OsUPS1GOS2) as well as silencing plants (OsUPS1RNAi). We found significant accumulation of allantoin in leaves, stems and roots of OsUPS1GOS2 while in OsUPS1RNAi allantoin was significantly accumulated in roots. We propose that OsUPS1 is responsible for allantoin partitioning in rice and its overexpression can support plant growth through accumulation of allantoin in sink tissues which can be utilized when N is limiting. © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
html
Digitial Object Identifier (DOI)
10.1111/pbi.13054
Recommended Citation
Redillas, M. R., Bang, S., Lee, D., Kim, Y., Jung, H., Chung, P., Suh, J., & Kim, J. (2019). Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions. Plant Biotechnology Journal, 17 (7), 1289-1301. https://doi.org/10.1111/pbi.13054
Disciplines
Biology
Keywords
Allantoin; Amino acids; Ammonium; Nitrogen; Rice
Upload File
wf_no