Autoflourescence study of root samples of Tsitsirika Catharanthus roseus (C. roseus)

College

College of Science

Department/Unit

Physics

Document Type

Archival Material/Manuscript

Publication Date

2003

Abstract

Images of the vinblastine-4'-anthranilate distribution on root samples of two variants (red flower and white flower) of C.roseus or Tsitsirika were obtained using an epi-fluorescence microscope (Olypmpus BX-51). Vinblastine-4'-anthranilate is fluorescent under UV irradiation with an excita- tion and emission wavelengths of 330 nm and 441 nm respectively. Twenty-five (25) different root samples for each variant were prepared which were laterally sectioned for larger image area. The root samples were placed on glass slide and imaged on the stage of the epi-fluorescence microscope. Images recorded by a sensitive CCD camera (Olympus DP-12) mounted on the trinocular port were processed in a desktop PC using Adobe Photoshop ver. 7.0 application program to calculate the histogram of the fluorescence image of vinblastine-4'-anthranilate. Keeping the optical settings of the microscope constant, t-test analysis of the mean luminosity showed that the active fluores- cent chemical (vinblastine-4'-anthranilate) was more apparent on the red variety than on the white variety of C.roseus.

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Disciplines

Physical Sciences and Mathematics | Physics

Keywords

Tsitsirika; Catharanthus roseus (C. roseus); Autofluorescence; Fluorescence microscopy; Plant physiology

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