Two-dimensional polystyrene microsphere tracking by back focal plane detection

Authors

C. S. Siton, De La Salle University, Manila
J. M. Yamson, De La Salle University, Manila
A. T. Ayen, De La Salle University, Manila
Romeric F. Pobre, De La Salle University, Manila

College

College of Science

Department/Unit

Physics

Document Type

Archival Material/Manuscript

Publication Date

2004

Abstract

A quadrant photodiode positioned at the back-focal plane of a microscope is used for the lateral (two-dimensional) position of polystyrene microsphere under a microscope glass slide. The computer software and hardware interface Science Workshop 750 supplied by PASCO, Inc. were used in gathering and evaluating data yielding calibration curves and micrometer measurements of actual position displacements of the particle. Resulting SNR values computed from the experiment range from 10 to 425. Furthermore, system resolutions for both x and y lateral positions were 1.051 volts/µm and 0.915-volts/µm respectively.

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Recommended Citation

Siton, C. S., Yamson, J. M., Ayen, A. T., & Pobre, R. F. (2004). Two-dimensional polystyrene microsphere tracking by back focal plane detection. Retrieved from https://animorepository.dlsu.edu.ph/faculty_research/12375

Disciplines

Physical Sciences and Mathematics | Physics

Keywords

Polystyrene; Particle tracking velocimetry; Particle tracks (Nuclear physics)

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