Date of Publication

2022

Document Type

Master's Thesis

Degree Name

Master of Science in Chemistry

Subject Categories

Chemistry

College

College of Science

Department/Unit

Chemistry

Thesis Advisor

Roger S. Tan

Defense Panel Chair

Rodolfo E. Sumayao, Jr.

Defense Panel Member

Aileen B. Angcajas
Emmanuel V. Garcia
Stephani Joy Y. Macalino

Abstract/Summary

Kidney malfunction is a common clinical condition and significant changes in normal kidney function are associated with chemotherapeutic drugs usage. Carboplatin is one of the most reliable antitumor agents against specific cancer types. Unfortunately, cancer patients are at high risk of developing drug-induced nephrotoxicity leading to death. Banaba is a known traditional herbal plant used by indigenous people for the treatment of illnesses. Anecdotal claims on L. speciosa leaf extract as a renal protective agent against Carboplatin toxicity are yet to be scientifically-validated.

Carboplatin was administered intraperitoneally (25 mg/kg) and L. speciosa extract (2g/100 ml) pre-treatment and post-treatment were administered orally. The treatments were subjected to different pharmacological activities including genotoxicity, hepatotoxicity and nephrotoxicity. L. speciosa extract reduced frequency of formation of micronucleus in the treated mice using micronucleus test. L. speciosa extract did not exhibit hepatotoxicity and it showed hepatoprotective effect by lowering ALT activity in pre-treatment and post-treatment groups. BAX expression was increased after Carboplatin exposure indicating increased mitochondrial stress. The extract reduced BAX expression in pre-treatment (p=0.001) and post-treatment (p=0.001) groups. The extract did not induce mitochondrial stress. Caspase-12 expression was increased after Carboplatin exposure indicating increased endoplasmic reticulum (ER) stress. However, it did not seem to mitigate Caspase-12 expression indicating the extract did not inhibit ER stress. In a separate study, L. speciosa extract exhibited analgesic activity secondary to Ibuprofen based on hot plate method. Preliminary investigation might support anecdotal claims on Banaba’s nephroprotective effect through suppression of BAX involved the intrinsic apoptosis pathway and attenuation of inflammatory effect.

Keywords: Lagerstroemia speciosa, Carboplatin, BAX, Caspase-12, apoptosis

Abstract Format

html

Language

English

Format

Electronic

Physical Description

[xxii], 132 leaves

Keywords

Antineoplastic agents; Apoptosis

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Embargo Period

3-18-2022

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