Date of Publication
11-2024
Document Type
Master's Thesis
Degree Name
Master of Science in Chemistry
Subject Categories
Chemistry
College
College of Science
Department/Unit
Chemistry
Thesis Advisor
Mariafe N. Calingacion
Defense Panel Chair
Emmanuel V. Garcia
Defense Panel Member
Raymond S. Malabed
Carlo Antonio T. Ng
Abstract/Summary
A steady rise in the popularity of the craft beers, which are artisanal beers produced by microbreweries, in the Philippines have been seen in recent years. With this rise in popularity, discussions have been brought up on what constitutes a craft beer, with many industry organizations having started efforts on conceptualizing such criteria. Here, the study hopes to contribute a scientific viewpoint on the topic via the analysis of craft beers from the Philippines. Chromatographic analyses via liquid chromatography-mass spectrometry were performed on craft beers, and in-processing craft beer samples in collaboration with a local craft brewery. Through statistical analyses on resulting profiles, metabolites were determined for 1) the differentiation of craft beers from their industrial counterparts, and 2) differential components within the craft brewing process for pathway analyses. Nineteen relevant markers were putatively identified for craft beer differentiation including 3-mercaptopyruvic acid, alongside 1-pentanethiol and humulinone for industrial beers. Pathway analyses through the brewing differential metabolites also revealed a significant association with the starch and sucrose metabolism, sulfur metabolism, and cysteine and methionine metabolism metabolite sets. The information provided from this study hopes to be of use for the Philippine craft beer industry to help further advance their development. Recommendations are suggested for 1) the further study of sulfur-based and humulinone-based targeted metabolomic approaches in craft beer, 2) the utilization of humulinone content and related metrics for the assessment of beer quality, and 3) further omics-based research on yeast sulfur metabolism, and studies on beer mashing, aging, and maturation.
Abstract Format
html
Language
English
Format
Electronic
Keywords
Beer--Philippines; Brewing; Alcoholic beverages
Recommended Citation
Carisma, N. S. (2024). Exploring the brewing craft: Characterizing Philippine craft beers through metabolomics-based approaches. Retrieved from https://animorepository.dlsu.edu.ph/etdm_chem/23
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Embargo Period
12-6-2026
