Date of Publication
2023
Document Type
Master's Thesis
Degree Name
Master of Science in Chemistry
Subject Categories
Chemistry
College
College of Science
Department/Unit
Chemistry
Thesis Advisor
Raymond S. Malabed
Ursela G. Bigol
Defense Panel Chair
Mariafe N. Calingacion
Defense Panel Member
Marissa G. Noel
Rosario S. Sagum
Abstract/Summary
Copra meal (CM) is an agricultural residue added to livestock feed formulations in the Philippines. However, it is added in small amounts as it contains anti-nutritional factors, mainly lignocellulose, and has insufficient protein content that affect the growth of livestock. CM was fermented with Aspergillus oryzae in order to increase its protein content and degrade lignocellulose. The parameters of solid state fermentation were optimized in this study to obtain the highest possible level of protein enrichment and to prepare for possible future scale-up processes. Box-Behnken design under the response surface methodology (RSM) was used to optimize pH (5-7), fermentation period (48 h – 144 h) and moisture content (50%- 70%) with crude protein enrichment (%) taken as the response to the design. The optimized parameters were a fermentation period of 95.33 h, moisture content of 58.49%, and pH of 6.1. Under such conditions, the crude protein enrichment was estimated to be 534.63%. Additionally, in fermented CM, crude fiber and in-vitro protein digestibility were decreased relative to unfermented CM. Thermogravimetric analysis (TGA) revealed the degradation of lignocellulose while the sugar profile analysis revealed the presence of glucose in fermented CM, whereas no glucose was detected in unfermented CM. Scanning electron microscopy (SEM) revealed the disruption of surface structure of CM after fermentation. Such findings suggest that the nutritional value of CM improved due to the increase in protein and the degradation of lignocellulose into simple sugars. These optimized parameters may also be considered for scaled-up production of protein-enriched CM for commercial livestock feed production.
Keywords: Aspergillus oryzae, copra meal, solid state fermentation, nutritional value
Abstract Format
html
Language
English
Format
Electronic
Keywords
Copra; Feeds; Aspergillus; Solid-state fermentation
Recommended Citation
Teh, J. T. (2023). Optimization of protein enrichment and enhancement of nutritional quality of copra meal fermented by aspergillus oryzae as potential animal feed Supplement. Retrieved from https://animorepository.dlsu.edu.ph/etdm_chem/15
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Embargo Period
12-3-2025
