Date of Publication

4-11-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Manufacturing Engineering and Management

Subject Categories

Biomedical Engineering and Bioengineering

College

Gokongwei College of Engineering

Department/Unit

Manufacturing Engineering and Management

Thesis Advisor

Joseph Rey H. Sta. Agueda

Defense Panel Chair

Nicanor R. Roxas, Jr.

Defense Panel Member

Homer S. Co

Catherine L. Ramos

Abstract (English)

Fish skin, although collagen-rich, is often discarded and considered as waste material. As such, this study repurposes invasive Chitala ornata (knifefish) skin by extracting its collagen and generating a valuable by-product to contribute to the advancement of wound dressing technology. Utilizing the extracted knifefish skin collagen, the manufacturing techniques of hydrogel production, 3D printing, and electrospinning were explored for the creation of a wound dressing material. Fourier-Transform Infrared Spectroscopy (FT-IR) confirmed the presence of characteristic collagen functional groups, indicating successful structure preservation. Thermogravimetric Analysis (TGA) found maximum thermal degradation of collagen-kappa-carrageenan (COLL-CARR) hydrogels to be at 259.77°C and electrospun collagen-polyvinyl alcohol (COLL-PVA) fibers to be at 304.66°C. Scanning Electron Microscopy (SEM) revealed that the electrospun fibers displayed porous and fibrous morphologies, while the hydrogel samples exhibited cracked and granular surfaces. Swelling and water retention tests on COLL-CARR hydrogels showed that the 2:1 formulation had the highest initial swelling capacity, while the 1:1 formulation showed more stable water retention. However, the 3D printed samples failed to maintain their form after extrusion, indicating insufficient structural integrity. COLL-PVA fibers, though successfully produced, dissolved rapidly in water due to the water-soluble nature of polyvinyl alcohol (PVA), making swelling and retention testing unfeasible. Through this study, it was found that COLL-CARR hydrogels with absorbent and water retention properties can be fabricated as a gel-like wound dressing while electrospun COLL-PVA fibers with a high surface area may be considered as a solid porous wound dressing option.

Abstract Format

html

Abstract (Filipino)

None

Abstract Format

html

Language

English

Format

Electronic

Keywords

Collagen

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Embargo Period

4-11-2026

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