Date of Publication

1-30-2026

Document Type

Dissertation/Thesis

Degree Name

Bachelor of Science in Chemistry

College

College of Science

Department/Unit

Chemistry

Thesis Advisor

Mariafe N. Calingacion 

Defense Panel Member

Aldrin P. Bonto 

Marissa G. Noel 

Abstract (English)

This study investigated the physical and chemical properties of extracted Adlay (Coix lacryma-jobi) oil from locally known soft variants, Gulian and Kiboa, and hard variants, S4 and S5. Soxhlet extraction was the method used to determine the oil yield using n-hexane as the solvent, with an average percent yield of ____. All four Adlay variants were quantitatively distinguished by measuring the cracking force (in Newtons) using the PASCO Material Testing Machine. The Adlay oil was also compared with a commercially available rice bran oil. The oil quality for each Adlay variant was assessed in oxidative stability by functional group characterization using using Attenuated Total Reflectance - Fourier Transformation Infrared (ATR-FTIR) spectroscopy. Phytochemical analysis was performed and quantified through total phenolic content (TPC), and total flavonoid content (TFC). The antioxidant activity was also evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to calculate for the percent radical scavenging activity (%RSA) and the free antioxidant activity. An intensity of ester carbonyl stretching attributed to triacyglycerols (TAGs) were relatively strong in Gulian and Kiboa, in comparison to S4 and S5, with S4 displaying a spectrum of high aldehyde and ketone carbonyl peaks. TPC concentration (in mg GAE/mL oil) and TFC concentration (in mg QE/mL oil) are notably highest in S4, followed by S5, Rice Bran, Kiboa, then the least for Gulian. Based on the free antioxidant activity and percent radical scavenging activity (%RSA), Kiboa had the highest antioxidant potential compared to other adlay variants and is comparable to the reference of Rice Bran oil. Statistical analysis revealed that there is a significant difference in phytochemical content between hard and soft adlay variants. Overall, findings highlight the clear physicochemical and antioxidant differences among the four Adlay variants.

Keywords: Adlay oil, cultivated adlay, wild adlay, Soxhlet extraction, phytochemical, antioxidant activity

Abstract Format

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Abstract (Filipino)

Sinuri sa pag-aaral na ito ang mga pisikal at kemikal na katangian ng langis mula sa Adlay (Coix lacryma-jobi) na nakuha mula sa mga lokal na malalambot na baryante (Gulian at Kiboa) at matitigas na baryante (S4 at S5). Ginamit ang Soxhlet extraction upang matukoy ang oil yield gamit ang n-hexane bilang solvent, na nagbigay ng karaniwang ani na 5.75% ± 0.021. Ang apat na baryante ng Adlay ay naiiba sa pamamagitan ng pagsukat ng cracking force (sa Newtons) gamit ang PASCO Material Testing Machine. Inihambing din ang langis mula sa Adlay sa isang komersiyal na rice bran oil bilang pamantayang sanggunian. Tinasa ang kalidad ng langis batay sa oxidative stability sa pamamagitan ng pagkilala sa mga functional group gamit ang Attenuated Total Reflectance–Fourier Transform Infrared (ATR-FTIR) spectroscopy. Isinagawa ang phytochemical analysis upang masukat ang total phenolic content (TPC) at total flavonoid content (TFC), habang sinuri naman ang antioxidant activity gamit ang 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay para sa pagtukoy ng percent radical scavenging activity (%RSA) at free antioxidant activity. Ipinakita ng mga resulta na ang ester carbonyl stretching intensity na iniuugnay sa triacylglycerols (TAGs) ay mas malinaw sa Gulian at Kiboa kumpara sa S4 at S5, kung saan ang S4 ay nagpakita ng mas mataas na mga peak ng aldehyde at ketone carbonyls. Ang konsentrasyon ng TPC (mg GAE/mL oil) at TFC (mg QE/mL oil) ay pinakamataas sa S4, sinundan ng S5, rice bran oil, Kiboa, at Gulian. Batay sa free antioxidant activity at %RSA, ang Kiboa ay nagpakita ng pinakamataas na antioxidant potential sa mga baryante ng Adlay at maihahambing sa rice bran oil. Ipinakita ng estadistikal na pagsusuri na may makabuluhang pagkakaiba sa phytochemical content sa pagitan ng mga malalambot at matitigas na baryante ng Adlay. Sa kabuuan, ipinapakita ng pag-aaral ang malinaw na pagkakaiba sa physicochemical properties at antioxidant activity ng apat na baryante ng Adlay, na nagbibigay-diin sa potensyal ng mga lokal na baryante bilang alternatibong pinagkukunan ng functional oil.

Keywords: langis mula sa Adlay, “cultivated” adlay, ligaw na adlay, “Soxhlet extraction”, “phytochemical, antioxidant activity”

Abstract Format

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Language

English

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Embargo Period

1-30-2026

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