Effect of different metal concentrations on ammonia detection by the salicylate method

Author

Mikkaela Denisse G. Futalan, De La Salle University, ManilaFollow
Joaquim Antonio V. Arquelada, De La Salle University, ManilaFollow

Date of Publication

8-2023

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biochemistry

Subject Categories

Biochemistry, Biophysics, and Structural Biology

College

College of Science

Department/Unit

Chemistry

Thesis Advisor

Laurenzo D.V. Alba

Defense Panel Member

Eric R. Punzalan
Lourdes P. Guidote

Abstract/Summary

The salicylate method is an established method used to quantify ammonia in a sample. The method involves the addition of a salicylate catalyst solution, an alkaline citrate solution, and an alkaline hypochlorite solution. All of which are involved in the Berthelot reaction that produces an indophenol-like dye, which can be quantitatively measured using a spectrophotometer. Giner-Sanz et al. (2020) has determined that iron interferes with the salicylate method, inhibiting the accurate measurement of ammonia. Thus, this study determined the effect of other transition metals to the salicylate method employed. Through a series of experiments, the study was able to determine that manganese, in the form of Mn²⁺, dramatically decreased the visible peak at 650 nm. With an R2 = 0.989, the TAN with Mn²⁺ standard is a good representation of the effect of Mn²⁺ to the indophenol reaction in the salicylate method. In addition, calculating for the equation at which the changes in absorbance is determined by the concentration of Mn²⁺ the Beer law has been used and the molar absorptivity was calculated at 27.7 mM-1 cm-1. In conclusion, the effect of Mn²⁺ can be quantified using the Beer’s law equation, and as such could be employed to determine the specific absorbance of TAN, in the presence of Mn²⁺ at predetermined concentration. While the salicylate method is used to determine the concentration of ammonia, the effect of Mn²⁺ was found to decrease the indophenol as the concentration of the metal increased, just like the known effect of Fe³⁺.

Abstract Format

html

Language

English

Format

Electronic

Keywords

Salicylates; Ammonia; Indophenol

Recommended Citation

Futalan, M. G., & Arquelada, J. V. (2023). Effect of different metal concentrations on ammonia detection by the salicylate method. Retrieved from https://animorepository.dlsu.edu.ph/etdb_chem/31

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Embargo Period

8-24-2024