Date of Publication

2024

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Gliceria B. Ramos

Abstract/Summary

S-100 proteins, unique to vertebrates, regulate cellular processes and serve as biomarkers for diseases. Rabbits, with its unique digestive system and well-researched anatomy, presents an ideal model for studying S-100 proteins and their role in gastrointestinal functions. While previous studies have investigated S-100 proteins in animals, no research has specifically examined their distribution in the rabbit gastrointestinal tract, highlighting a gap that could provide valuable insights into their physiological and pathological roles. With the aim to distinguish presence of S-100 protein in the stomach (cardia, fundus, pylorus), small intestine (duodenum, jejunum, ileum), and large intestine (cecum, colon, rectum), the researchers target to compare such presence between male and female rabbits. S-100 protein presence was determined using immunohistochemistry in various gastrointestinal tract sections of 6 (3 male and 3 female) rabbits. Samples were taken from the stomach, small intestine, and large intestine for the immunohistochemical detection of the target protein. S-100 proteins were primarily detected in the lining epithelium, lamina propria, and muscularis mucosae across all regions. In the stomach, S-100 was most evident in the lining epithelium and the lamina propria, with some variations in the basal cytoplasm and blood vessels of the fundus and pylorus regions, respectively. In the small intestine, S-100 proteins were detected mainly in the columnar epithelial cells and Paneth cells, while goblet cells showed no immunoreactivity. In the large intestine, S-100 proteins were present in the epithelial lining and blood vessels, particularly in the cecum and colon. The immunoreactivity of S-100 proteins in the different gastrointestinal regions suggests their role in maintaining epithelial integrity and modulating the immune response. This study contributes to the understanding of S-100 protein distribution in rabbits and provides a basis for further research into their role in gastrointestinal health and disease.

Abstract Format

html

Language

English

Format

Electronic

Keywords

Immunohistochemistry; Gastrointestinal system; Rabbits—Anatomy; Proteins—Analysis

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Embargo Period

12-5-2024

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