Isolation and identification of yeasts found in selected urban wastewater and assessment for in vitro antimicrobial resistance

Date of Publication

8-2024

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Microbiology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Llewelyn Moron-Espiritu

Defense Panel Member

Christian Jordan O. Dela Rosa
Florabelle D. Querubin

Abstract/Summary

The prevalence of antimicrobial resistance is quickly becoming a global health crisis, especially due to humans’ constant exposure to different pathogenic microorganisms, including yeasts. With this, further studies on antimicrobial resistance are important in determining effective prevention and treatment of infections caused by such pathogens. This study aimed to assess the antimicrobial susceptibility against azole drugs of yeasts isolated from urban wastewater samples. In addition, identification of the yeast isolates in wastewater was also conducted using phenotypic methods such as Gram staining and germ tube assay, as well as genotypic methods through molecular sequencing. A total of 20 presumptive yeast colonies based on colony morphology and microscopic analysis were isolated and further purified. Sequencing of the Internal transcribed spacer (ITS) 4 and 5 regions for molecular sequence analysis confirmed the following species: (i) Candida glabrata, (ii) Candida tropicalis, and (iii) Candida nivariensis. Results of the antimicrobial susceptibility testing revealed that five isolates were susceptible-dose-dependent (SDD) and fifteen isolates were susceptible to both fluconazole (25 ug) and voriconazole (1 ug). This study sheds light on the current antimicrobial resistance patterns of yeast species in wastewater collected from DLSU-Manila.

Keywords: Urban wastewater, antimicrobial resistance, Candida glabrata, Candida tropicalis, Candida nivariensis, voriconazole, fluconazole

Abstract Format

html

Language

English

Format

Electronic

Keywords

Sewage—Microbiology; Anti-infective agents; Candida tropicalis; Yeast

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Embargo Period

8-12-2024

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