Date of Publication

11-2024

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Maria Rowena R. Romana-Eguia

Defense Panel Chair

Maria Carmen A. Lagman

Defense Panel Member

Biena Marie S. Joaquin
Jandeil B. Roperos

Abstract (English)

Increase in global temperature due to climate change affects several sectors of the economy, including alterations to aquatic environments that potentially affect the physiology and survival of fish species. This study investigates the effects of acute heat changes and salinity conditions based on gene expressions. Moreover, the study specifically evaluates  effects of the aforementioned parameters by quantifying total soluble muscle protein concentrations in Chanos chanos (Milkfish) using the Bradford Assay and by determining and comparing protein expression profiles through Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The experimental groups are exposed to increasing temperatures. In a separate, preliminary experiment, milkfish Chanos chanos juveniles were acclimated for ten days in their respective aquariums based on salinity levels of freshwater, brackish water, and sea water. Moreover, the experimental samples were subjected to increasing temperatures from 26-32°C, while the ambient temperature remained at a constant room temperature as the control. For this study, ten muscle samples were collected across all treatments, amounting to 60 samples with three replicates per sample. These samples were collected with a phosphate-buffered saline (PBS)-based protocol for protein profiling. The Bradford Assay results indicated sufficient protein content across all samples. In SDS-PAGE, it was revealed that salinity reinforced the adaptability of Chanos chanos to maintain homeostasis. It was found that milkfish was able to modulate proteomic responses to maintain homeostasis. However, the temperature indicated biochemical response to acute thermal stress conditions due to the presence of altered protein bands. Furthermore, these findings indicated the tolerance of Chanos chanos to environmental variation.

Abstract Format

html

Abstract (Filipino)

Ang pagtaas ng temperatura ng mundo ay nakakaapekto sa mga ilang sektor ng ekonomiya, isa na rito ay ang pagbabago ng estado ng kapaligiran sa tubig na posibleng makaapekto sa pisyolohiya at kaligtasan ng mga iba’t ibang species ng isda. Sinisiyasat ng pag-aaral na ito ang mga epekto ng matinding pagbabago sa init at mga kondisyon ng kaasinan batay sa gene expression. Bukod dito, partikular na sinusuri ang mga muscle tissues ng mga Chanos chanos (milkfish) at ang mga epekto sa Bradford Assay upang malaman ang konsentrasyon ng protina at pagtukoy at paghahambing ng mga protein expression sa pamamagitan ng Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Gayunpaman, ang mga nakolektang isda ay na-acclimate sa loob ng sampung araw sa kani-kanilang aquarium batay sa antas ng kaasinan ng freshwater, brackish water, at sea water. Ang mga eksperimentong sample o heated ay sumailalim sa pagtaas ng temperatura mula 26–32 °C, habang ang mga kontrol ay nanatiling pare-parehong temperatura o ambient ng kanilang aquarium. Mayroong 10 mga sample ng kalamnan na nakaloketa batay sa mga treatment na umabot sa 60 sample na may tatlong mga replika bawat sample. Ang mga sample na ito ay nakaloketa gamit ang isang  phosphate-buffered saline (PBS)-based protocol. Ang mga resulta ng Bradford Assay ay nagpahiwatig ng sapat na nilalaman ng proteina sa lahat ng mga sample. Sa SDS-PAGE, ipinahayag na ang kaasinan ay nagpatibay sa kakayahang umangkop ng Chanos chanos upang mapanatili ang homeostasis. Gayunpaman, ang temperatura ay nagpahiwatig ng biochemical na reaksyon sa talamak na mga kondisyon ng thermal stress dahil sa pagkakaroon ng mga pagbabago sa protein bands. Samakatuwid, ang mga Chanos chanos ay patunay na umaangkop sa pagbabago ng kapaligiran.

Abstract Format

html

Language

English

Format

Electronic

Keywords

Milkfish; Fishes—Effect of temperature on

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Embargo Period

4-20-2027

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Available for download on Tuesday, April 20, 2027

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