Date of Publication

7-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology major in Medical Biology

Subject Categories

Food Microbiology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Florabelle D. Querubin

Defense Panel Chair

Christian Jordan O. Dela Rosa

Defense Panel Member

Paul Benedic U. Salvador
Kris Lord T. Santos

Abstract (English)

This study evaluates the gastrointestinal viability of Lactobacillus casei strain Shirota (LcS) by integrating a systematic review and an in vitro simulation. The systematic review (2014–2024) identified LcS survival mechanisms under gastrointestinal (GI) stress, including resistance to acid and bile and improved performance under reduced gut motility. These insights guided the experimental design simulating stomach phases under both normal and hypoperistaltic conditions. The simulations involved exposure to acidic pH (as low as pH 2.0), pepsin (25 mg/mL), pancreatin (2 g/L), and bile salts (12 g/L). Results showed minimal viability in gastric conditions but significantly improved viability in duodenal and ileal phases under hypoperistalsis, likely due to reduced mechanical agitation. The findings confirm that slowed transit enhances LcS viability and underscore the relevance of combining literature-based insights with physiologically relevant models to better understand probiotic performance across digestive environments.

Keywords: Lactobacillus casei Shirota, gastrointestinal simulation, probiotic viability, digestive stress, bile salt viability, hypoperistalsis model

Abstract Format

html

Abstract (Filipino)

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Abstract Format

html

Language

English

Format

Electronic

Keywords

Lactobacillus casei; Gastrointestinal system; Probiotics; Fermented beverages; Bile salts

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Embargo Period

8-11-2028

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Available for download on Friday, August 11, 2028

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