Date of Publication
7-2025
Document Type
Bachelor's Thesis
Degree Name
Bachelor of Science in Biology major in Medical Biology
Subject Categories
Molecular, Cellular, and Tissue Engineering
College
College of Science
Department/Unit
Biology
Thesis Advisor
Mariquit M. De Los Reyes
Defense Panel Chair
John Martin S. Mondragon
Defense Panel Member
Gerard Anthony M. Espiritu
Tosha Mae S. Manalastas
Abstract (English)
This study aimed to refine a novel decellularization protocol for porcine skin by evaluating the effects of isopropanol delipidation as the first step to decellularization, combined with varying duration and wattages of ultrasonic treatment (sonication). Delipidized samples were subjected to decellularization at 0 W, 180 W, and 240 W for 3, 6, and 9 hr. After treatment, samples underwent histological analysis with a standardized scoring sheet evaluated by 11 independent scorers. Samples treated at 240 W were consistently found to achieve high scores across two independent runs, indicating effective removal of cellular components while preserving extracellular matrix (ECM) integrity. Further analysis involving Scanning Electron Microscopy (SEM) showed 240 W, 6 hr and 9 hr samples with well-defined fibrous structures resembling collagen. Elemental analysis through EDS, including both quantitative and count mapping, confirmed the consistent presence of carbon, oxygen, and nitrogen, suggesting successful retention of proteinaceous components. ATR-FTIR spectroscopy complemented these observations through the identification of key functional groups associated with collagen (Amide I, II, III) and lipid degradation through reduced CH₂ and CH₃ peaks. The results validate the improved effectiveness of the protocol in decellularizing porcine epidermis while preserving critical ECM structures, highlighting its potential for future tissue engineering applications.
Abstract Format
html
Abstract (Filipino)
"-"
Abstract Format
html
Language
English
Format
Electronic
Keywords
Tissue engineering; Extracellular matrix
Recommended Citation
Basila, M. S. (2025). Decellularization of porcine skin for the isolation of extracellular matrix (ECM) for tissue engineering applications. Retrieved from https://animorepository.dlsu.edu.ph/etdb_bio/144
Upload Full Text
wf_yes
Embargo Period
8-12-2026
