Date of Publication

4-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

Marigold O. Uba

Defense Panel Member

Michael B. Ples
Patricio Elvin D. Cantiller
Christian Jordan O. Dela Rosa

Abstract (English)

Antimicrobial resistance (AMR) in Enterobacteriaceae is a growing health concern, particularly in poultry farms where resistant bacteria can spread to humans. The study examines the antibiotic resistance of Enterobacteriaceae species in 12 chicken stool samples collected from two poultry farms—Small (SF) Farm and Big (BF) Farm in Barangay Sumandig, San Ildefonso, Bulacan. Bacterial identification was performed using biochemical tests such as IMViC tests, as well as selective and differential agar media. In SF, Salmonella spp. was most prevalent, with 50.0% (6/12) of the isolates. Followed by Escherichia coli and Providencia spp., each representing 16.7% (2/12). Proteus spp. was least common, making up 8.3% (1/12) of the isolates. In BF, Escherichia coli was prevalent with 53.8% (7/13). Salmonella spp. and Serratia spp. made up 15.4% (2/13) each out of the total isolates. Providencia spp. and Enterobacter spp. accounted for 7.7% (1/13) each, making them least common. Antimicrobial susceptibility was assessed using Kirby-Bauer disk diffusion method and antibiotics tested were Ampicillin (AMP), Meropenem (MEM), Ciprofloxacin (CIP), Doxycycline (DOX), Trimethoprim-Sulfamethoxazole (TMP-SMX), and Chloramphenicol (CHL). Results revealed the presence of multiple Enterobacteriaceae species with varying resistance against the antibiotics. Notably, Doxycycline resistance was prevalent in both farms, 91.6% (11/12) from SF and 84.6% (11/13) from BF were found to be resistant to Doxycycline. High resistance to β-lactams and Tetracyclines suggest excessive antibiotic use in poultry farming. This study underscores the need for improved antibiotic management, farm hygiene, and policies regulating antibiotic use. The findings offer valuable insights into AMR in local poultry farms and its potential impact on public health.

Abstract Format

html

Abstract (Filipino)

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Abstract Format

html

Language

English

Format

Electronic

Keywords

Enterobacteriaceae; Poultry--Philippines--Bulacan; Stools; Feces—Microbiology; Drug resistance in microorganisms; Multidrug resistance

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Embargo Period

4-12-2027

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Available for download on Monday, April 12, 2027

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