Date of Publication

4-2025

Document Type

Bachelor's Thesis

Degree Name

Bachelor of Science in Biology

Subject Categories

Biology

College

College of Science

Department/Unit

Biology

Thesis Advisor

John Martin S. Mondragon

Defense Panel Chair

Mariquit M. De Los Reyes

Defense Panel Member

Gerard Anthony M. Espiritu
Tosha Mae S. Manalastas

Abstract (English)

Decellularization is a viable strategy for creating extracellular matrix (ECM) scaffolds for skin substitutes. The ECM, located within the epidermis, is essential for tissue structure, repair, and function. Among animal sources, porcine skin is a viable and comparable source as a potential decellularized scaffold. This study evaluated the hybrid decellularization method for porcine skin through the use of hypotonic and hypertonic solutions subjected to three different temperature treatments (2-4°C, 22°C, and 37°C). This approach aimed to eliminate cellular components while preserving the structural integrity and composition of the native ECM without using conventional detergents. The decellularized extracellular matrix (dECM) was evaluated through macroscopic evaluation and assessment of decellularization efficiency through Hematoxylin and Eosin (H&E) staining, Scanning Electron Microscopy (SEM), and Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy (ATR-FTIR). Macroscopic evaluation showed a gradual color change into translucent white tissue, exhibiting decellularization within 7 to 9 hours. H&E staining analysis revealed that higher temperatures resulted in better cell removal. Although the highest temperature caused moderate damage, a visibly intact ECM structure. While for SEM, the highest temperature resulted in enhanced cell removal efficiency; however, the preservation of the ECM structure was compromised. Spectral analysis shows a difference in the chemical structure and composition of the native skin and decellularized porcine skin. The presence of structural components specific within the ECM suggests the preservation of tissue composition. These preliminary results demonstrate the feasibility of a detergent-free and temperature-dependent approach that can provide efficient cell removal and preservation of the ECM integrity. Notably, this method offers a safer alternative for porcine tissue decellularization, as it eliminates the use of harsh detergents posed by its cytotoxic risks and potential tissue damage. By reducing effects associated with detergent-based methods, this approach increases the suitability for biomedical applications. The findings may contribute to ongoing research to improve the approaches for the development of skin substitute materials.

Abstract Format

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Abstract (Filipino)

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Abstract Format

html

Language

English

Format

Electronic

Keywords

Tissue engineering; Extracellular matrix

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Embargo Period

4-13-2026

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Available for download on Monday, April 13, 2026

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