Date of Publication

3-4-2010

Document Type

Master's Thesis

Degree Name

Master of Science in Chemistry

Subject Categories

Chemistry

College

College of Science

Department/Unit

Chemistry

Thesis Adviser

Consolacion Y. Ragasa

Defense Panel Chair

Jaime Raul O. Janairo

Defense Panel Member

Glenn V. Alea
Ma. Alicia M. Aguinaldo

Abstract/Summary

The dichloromethane extract of the air-dried leaves of Bixa orellana L. afforded ishwarane, phytol, polyprenol, and a 1:3 mixture of β-stigmasterol and β-sitosterol by silica gel chromatography. The structure of ishwarane was elucidated by extensive 1D and 2D NMR spectroscopy. It was also tested for analgesic, hypoglycemic, antispasmodic, and prophylactic activity at three dosages (25, 50 and 100 mg/kg BW) and for antimicrobial potential.

Results of the prophylactic assay demonstrated the anti-toxic property of ishwarane at 100 mg/kg BW where signs of recovery were already evident at 2.5 hours after treatment administration, while the negative control showed signs of recovery only at 5 hours postreatment. The 50 mg/kg BW ishwarane (88.38±13.59%) resulted to a more propulsive movement of the gastrointestinal tract compared to the negative control (78.47±10.61%) which might indicate a possible cholinergic activity. It did not, however, exhibit any hypoglycemic activity or analgesic potential using the acetic acid writhing assay. Ishwarane exhibited moderate antifungal activity against C. albicans (AI = 0.3), low activity against T. mentagrophytes (AI = 0.3) and low antibacterial activity against E. coli (AI = 0.1), S. aureus ( AI = 0.1) and P. aeruginosa (AI = 0.3). It was inactive against B. subtilis and A. niger.

Abstract Format

html

Language

English

Format

Electronic

Accession Number

CDTG004692

Shelf Location

Archives, The Learning Commons, 12F Henry Sy Sr. Hall

Physical Description

148 leaves ; 28 cm.

Keywords

Annatto tree—Analysis; Foliar diagnosis; Sesquiterpenes

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